烟酰胺对长波紫外线照射黑素细胞黑素合成的干预  

Intervention of nicotinamide on skin melanin genesis after UVA exposed

作　　者：帕它木.莫合买提 金锡鹏[2] 张玉彬[2] 潘建英[2] 沈光祖[2] 

机构地区：[1]新疆医科大学公共卫生学院劳动卫生与环境卫生学教研室,乌鲁木齐830054 [2]复旦大学公共卫生学院皮肤生理毒理学研究室,上海200032

出　　处：《中华劳动卫生职业病杂志》2007年第8期465-469,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：新疆维吾尔自治区科技厅资助(200623113)

摘　　要：目的研究烟酰胺对长波紫外线(UVA)致人皮肤黑素细胞黑素合成的干预作用。方法观察0.2J/cm^2 UVA(365 nm)照射人皮肤黑素细胞后,烟酰胺在不同剂量时对人皮肤黑素细胞中黑素合成和转运的干预作用。结果0.2J/cm^2UVA照射后,黑素细胞中黑素含量明显增加,UVA照射后的人皮肤黑素细胞给予不同剂量的烟酰胺时,黑素细胞中黑素含量明显下降,10.0mmol/ml时作用更为明显。烟酰胺在有效抑制黑素含量的同时,对黑素细胞的细胞周期、细胞凋亡及DNA指数无明显影响;0.2J/cm^2 UVA照射后立即给予烟酰胺时,烟酰胺可以调节黑素细胞中mRNA的表达水平。结论烟酰胺能够拮抗UVA的致黑作用;10.0mmol/ml烟酰胺在干预UVA所致的黑素细胞致黑作用的同时,对黑素细胞的作用浓度是安全的;烟酰胺参与其中黑素的转运;鉴于烟酰胺拮抗UVA致黑作用的有效结果,烟酰胺有望用于防护UVA照射所引起的晒黑作用。Objective To investigate the interference effect of nicotinamide on UVA-induced melanin genesis and melanin transport in human skin melanocyte. Methods The optimum UVA dose expected to cause cell proliferation： 0.2 J/cm^2, nicotinamide was added immediately after the 0.2 J/cm^2 UVA exposure and the melanin content, cell cycles, cell apoptosis and mRNA express level were measured respectively. Results Melanin content in melanocytes was increased significantly after exposed to 0.2 J/cm^2 UVA. Melanin content in melanocytes was decreased after treatment with 10.0 mmol/ml nicotinamide following UVA exposure,but the cell cycles and the cell apoptosis rate were not significantly altered, mRNA express levels of TYR,TRP-1 were modulated by nicotinamide. Conclusion Nicotinamide has more effect on decreasing melanin genesis after UVA exposure; nicotinamide also plays a role in modulating the mRNA express of TYR, TRP-1 gene. It is possible to consider nicotinamide as an efficient and safe sun screen to provide a certain level of protection for UVA exposed skin.

关 键 词：烟酰胺 紫外线 黑素细胞 干预性研究 

分 类 号：R751[医药卫生—皮肤病学与性病学]