转BADH、mtlD基因旱稻耐盐性比较  

Comparison of the Salt-tolerance of Upland Rice Transferred with BADH and mtlD Genes

作　　者：王智华[1] 尹凤川[1] 张凌云[1] 徐德芳[1] 

机构地区：[1]东营职业学院,山东东营257091

出　　处：《安徽农业科学》2007年第24期7401-7402,共2页Journal of Anhui Agricultural Sciences

基　　金：国家转基因植物研究与产业化开发专项(JY03-13-10)

摘　　要：为了获得变异材料，培育耐盐水稻，以便更好地开发利用盐渍耕地。将转入BADH和mtlD基因的5个旱稻新品系在黄河三角洲盐渍土上种植。通过产量及主要农艺性状的比较，分析转基因旱稻的耐盐性差异，选出耐盐能力强的品系并确定其耐盐范围。在含盐量（0.30±0.05）％土壤种植旱稻，2号品系较对照增产最少，为60kg/hm^2；5号品系较对照增产最多，为420kg/hm^2。在含盐量（0.50±0.05）％土壤种植旱稻，供试品系较对照增产270-1440/hm^2，5号品系增产最多，为33.3％。在含盐量（0.80±0.05）％土壤种植旱稻，各品系较对照增产7.5％-52.7％；3号品系增产最少，为2210kg/hm^2；5号品系增产最多，达1470kg/hm^2。在不同含盐量地块各供试品系在产量及农艺性状上均有较大差异，其中99-4、99-5两品系耐盐渍能力较强，适宜耐盐范围为（0.50±0.05）％。The study aimed to obtain genetic variation material and breed salt-tolerant rice varieties so as to develop and utilize salinized cultivated-land better.5 new lines of upland rice transferred with BADH and mtlD genes were planted in the salinized land in the Yellow River Delta. Through the compar-ison of the yield and major agronomy traits, the salt-resistance difference of transgenic upland rice was analyzed and the lines with strong salt-tolerance were screened and their salt-tolerance range was confmned.Compared with CK, when the upland rice were planted in the salinized land with salt content at （0.30±0.05） %, line 2 increased leastyield, being 60 kg/hm^2 and line 5 increased most, being 420 kg/hm^2 ; when the upland rice were planted in the salinized land with salt content at （0.50±0.05） %, the test line increased 270 - 1440 kg/hm^2 and line 5 increased most, being 33.3 % ; when the upland rice were planted in the salinized land with salt content at （0.80±0.05） %, each line increased 7.5 %-52.7%, line 3 increased least, being 210 kg/hm^2 and line 5 increased most, being up to 1 470 kg/hm^2.Each tested lines had great difference in yield and agronomy traits in the land with different salt contents, in which, line 99-4 and 99-5 had stronger sah-tolerance and their suitable sah-tolerance range was （0.50±0.05）%.

关 键 词：BADH MILD 旱稻 耐盐 产量 农艺性状 

分 类 号：S511[农业科学—作物学]