费氏中华根瘤菌ntrBC基因突变株的构建及其特性  

Construction and Characterization of ntrB^- and ntrC^- Mutants of Sinorhizobium fredii

作　　者：龚焘[1] 陆祖军[1] 陈钢[1] 曹永强[1] 武波[1] 

机构地区：[1]广西大学生命科学与技术学院微生物及植物遗传工程教育部重点实验室,广西亚热带生物资源保护利用重点实验室,南宁530005

出　　处：《应用与环境生物学报》2007年第4期523-525,共3页Chinese Journal of Applied and Environmental Biology

基　　金：国家973计划(No.001CB108901);国家自然基金资助项目(No.30570048)~~

摘　　要：从费氏中华根瘤菌(Sinorhizobium fredii)HN01基因文库的重组质粒pGXHN300上获得ntrB、ntrC基因完整序列.通过自杀质粒pK18mob同源单交换的方法,分别获得了HN01菌株的ntrB和ntrC基因的突变株GXHNB、GXHNC.功能检测证明GXHNB、GXHNC能以硝酸盐为唯一氮源进行正常生长,但不能以铵源作为唯一氮源进行正常生长.The ntrB and ntrC genes of Sinorhizobium fredii strain HN01 were obtained from recombinant plasmid pGXHN300. The ntrB^- and ntrC^- mutants, named GXHNB and GXHNC, were constructed through homologous recombination caused by a single crossover event. Compared with parent strain HN01, the GXHNB and GXHNC were able to grow on the medium with nitrate as the sole nitrogen source, but grew weakly on the medium with ammonium as the sole nitrogen source. Fig 4, Tab 1, Ref 15 .

关 键 词：费氏中华根瘤菌 ntrB基因 ntrC基因 突变体 

分 类 号：S154.3[农业科学—土壤学]