山萘酚的电化学行为及其电极反应机理研究  

Electrochemical behavior of Kaempferol and its reaction mechanism on a glassy carbon electrode

作　　者：刘爱林[1] 张少波[1] 吴潇霎[1] 李丽萍[1] 曹扬远[1] 林新华[1] 

机构地区：[1]福建医科大学药学院药物分析系,福州350004

出　　处：《海峡药学》2007年第8期36-39,共4页Strait Pharmaceutical Journal

基　　金：国家863计划项目(2006AA022421);国家自然科学基金资助项目(20675015);福建省科技重点基金资助项目(200610016);福建省青年人才基金资助项目(2006F3058)

摘　　要：目的建立电化学检测山萘酚含量的方法。方法本文采用伏安法研究山萘酚在玻碳电极上的电化学行为及其电极反应机理。在磷酸盐缓冲液中(pH5.0),在-0.2～+0.8V范围内山萘酚在玻碳电极表面是受吸附控制,发生准可逆单电子转移电极反应过程,电子转移系数为α=0.467;并以差示脉冲伏安法建立了检测山萘酚含量的新方法。结果山萘酚在玻碳电极上有一对氧化还原峰,峰电位Epa=0.401V,Epc=0.347V,测得其氧化峰电流Ip与浓度1.0×10-7～5.0×10-6mol.L-1范围内成良好的线性关系,检出限为1.0×10-8mol.L-1。结论本法操作简单、快速、灵敏、准确,可为山萘酚药物质量的控制和检测提供了一种简便的方法。OBJECTIVE To develop a new method for the determination of Kaempferol. METHODS The electrochemical behavior of Kaempferol was studied by voltammetry with a glassy carbon electrode. The results exhibited the well - defined redox peak of Kaempferol was observed and the electrode process was adsorption - controlled. The charge transfer coefficient （α） was calculated as 0. 467 in phosphate buffer solution （pH 5.0 ） . RESULTS The relationships between oxidation peak current and the concentration of Kaempferol were linear in the range of 1.0 × 10 ^-7 - 5.0× 10^ -6 tool · L^-1 by Differential Pulse Vohammetric method. The detection limit had been estimated as 1.0 × 10^-6 mool ·L^-1. CONCLUSION This method an be used for the determination of Kaempferol with satisfactory results.

关 键 词：山萘酚 电化学行为 伏安法 黄酮类化合物 

分 类 号：R927.2[医药卫生—药学]