棉浆黑液生物处理系统中嗜碱细菌的多样性  

Diversity of Alkaliphiles from the Black Liquor of Cotton Pulp Treatment System

作　　者：文金丽[1] 缪礼鸿[1] 王璐[1] 谢福莉[2] 李友国[2] 姚斌[3] 

机构地区：[1]武汉工业学院生物与制药工程系,湖北武汉430023 [2]华中农业大学生命科学技术学院/农业微生物国家重点实验室,湖北武汉430070 [3]中国农业科学院饲料研究所,北京100081

出　　处：《武汉大学学报（理学版）》2007年第4期491-496,共6页Journal of Wuhan University:Natural Science Edition

基　　金：国家高技术研究发展计划(863)项目(2004CB719606);农业微生物国家重点实验室开放课题经费资助

摘　　要：以棉浆黑液为细菌分离培养基,从某化纤厂黑液生物处理系统中分离到16株嗜碱细菌,对其形态及生理生化等特征的研究结果表明,所分离的嗜碱细菌以产芽孢的革兰氏阴性细菌占优势,在个体形态和生理生化特性方面具有丰富的多样性.扩增rDNA限制性分析(ARDRA)结果表明,棉浆黑液中的嗜碱细菌分为2大类群,不同菌株之间的酶切图谱具有较大的差异.测定黑液生物处理系统中6株占优势的嗜碱细菌的16S rDNA序列,与国际上已报道的细菌的16S rDNA序列进行比较,结果表明:它们的序列与芽孢杆菌属(Bacillus)或副球菌属(Paracoccus)中的参比菌株的同源性达到98%以上.以16S rDNA序列为基础的Bacillus属嗜细菌的系统发育分析结果显示,其中3个菌株与B.halodurans和B.wakoensis的相似性大于99%.Sixteen alkaliphilic strains were isolated from pulp wastewater of a fiber factory. The morphology and phenotypic characteristics showed that these trains displayed great diversity. Those strains of the gram-negative alkaliphilic bacteria producting spore were dominant. Those strains were classed into two families by amplified rDNA restriction analgsis （ARDRA） and 16S rDNA sequences, their fingerprinting patterns shows significant differences. The homology of six strains tested achieves above 98% on 16S rDNA sequences comparing to Bacillus and Paracoccus species which had reported, and the homology of three strains achieves above 99% with B. halodurans and B. wakoensis.

关 键 词：棉浆黑液 嗜碱细菌 生物多样性 

分 类 号：Q938[生物学—微生物学]