SPE-HPLC测定食品中芍药苷的含量  

Determination of Paeoniflorin in Food by HPLC

作　　者：颜红[1] 彭富全 李晓瑜[3] 

机构地区：[1]广东省药品审评认证中心,广东广州510080 [2]广州陈李济药厂,广东广州510290 [3]广州市医药中专学校,广东广州510430

出　　处：《现代食品与药品杂志》2007年第4期42-44,共3页JOurnal of Modern Food and Pharmaceuticals

摘　　要：目的建立一种测定食品中芍药苷含量的方法。方法用SPE提取芍药苷,绘制流出曲线,用高效液相色谱法定量,采用HP ZOBAX SB C18型色谱柱,甲醇∶水∶磷酸(20∶80∶0.05)为流动相,检测波长230 nm。结果线性范围为0.0365～0.365μg/ml,γ=0.9995,回收率为94.7%,RSD为2.0%。结论本法操作简便,结果准确,可作为食品中芍药苷含量测定的参考方法。Objective To establish a SPE-HPLC method to analyze paeoniflorin in food. Methods Paeoniflorin was extracted from food by solid-phase extraction. HP ZOBAX SB C18 column with SPE was used and the mobile phase was composed of solution CH3OH-H2O-H3PO4 （20： 80：0.05 ）. UV detective wavelength was set at 230 nm. Results The linear range of paeoniflorin in food was 0. 0365 - 0. 365 μg（ r = 0. 9995 ）. The average recovery was 94.7 % and RSD was 2.0%. Conclusion This method is easy, accurate and it can be used for quality control of the paeoniflorin in food.

关 键 词：SPE HPLC 芍药苷 

分 类 号：R284.1[医药卫生—中药学]