ENR基因在高油和普通玉米自交系中的克隆和表达分析  

The Cloning and Expression Analysis of ENR Gene of High-Oil and Normal Inbred Lines in Maize

作　　者：柳展基[1] 唐桂英[1] 单雷[1] 毕玉平[1] 

机构地区：[1]山东省农科院高新技术研究中心山东省作物与畜禽品质改良生物技术重点实验室,山东济南250100

出　　处：《华北农学报》2007年第4期1-4,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(30571165)

摘　　要：利用高油玉米自交系By804和普通玉米自交系B73为材料,克隆Enoyl-ACP reductase(ENR)基因,同时比较ENR基因的序列和表达差异。结果表明,ENR基因编码蛋白在高油和普通玉米中氨基酸序列完全一致;在授粉后15,25和35 d的胚中,ENR基因呈下调表达,在同一发育时期,ENR基因在高油玉米By804中较高表达。High-oil inbred line By804 and normal inbred line B73 were utilized to clone the gene of enoyl-ACP reductase（ENR） and then to discriminate the difference of sequence and expression at RNA level of ENR in By804 and B73. The results indicated there was no difference in amino acid sequence of ENR in By804 and B73 ; the expression at RNA level was down-regulated at 15,25 and 35 d after pollination（DAP） in both By804 and B73 and the expression of ENR in By804 was higher than that in B73 at the same embryo development stage.

关 键 词：高油玉米 ENR基因 表达分析 

分 类 号：S513.03[农业科学—作物学]