水稻粳型亲籼系S-c座位基因型分析  

Genotypic Identification of the S-c Locus in the Indica-compatible Japonica Lines of Rice(Oryza sativa L.)

作　　者：丁效华[1] 张泽民[1] 曾瑞珍[1] 张桂权[1] 

机构地区：[1]华南农业大学广东省植物分子育种重点实验室,广东广州510642

出　　处：《华北农学报》2007年第4期5-8,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金(30471068);广东省科学技术项目资助(2004B20101003)

摘　　要：S-c是控制水稻籼粳亚种间杂种F1花粉不育性的基因座位之一。在该座位,台中65的基因型为Sj/Sj,广陆矮4号的基因型为Si/Si。以台中65和广陆矮4号为遗传测验种,分别与4个粳型亲籼系配组,根据部分F2群体中植株花粉育性表型及与S-c紧密连锁分子标记基因型的偏态分离程度,测定了这4个粳型亲籼系在S-c座位的基因型,结果表明,G2416-3的基因型为Si-2/Si-2;G2605和G3004-4的基因型均为Si-1/Si-1;G2417-2-1的基因型为Sn/Sn。本文还对F1花粉不育性基因遗传分化的测定方法进行了讨论。S-c is one of the F1 pollen sterility loci between indica and japonica cultivars in rice. The genotype of Taichong 65（T65） and Guangluai 4（GLA 4） at the locus is S^j/S^j and S^i/S^i, respectively. Four indica-compatiblejaponica lines（ICJLs）,G2416-3,G2417-2-1 ,G2605 and G3004-4 were crossed with testers,T65 and GLA 4,and their S-c locus genotypes were identified. The segregation of PCR-based molecular markers linked tightly with S-c locus and plant pollen fertility in several F2 populations showed that G2416-3 possessed S^i-2/S^i-2 genotype; G2605 and G3004-4 possessed S^i - 1/S^i- 1 genotype; while G2417-2-1 possessed S^n/S^n genotype. The test methods on genetic differentiation of the F1 pollen sterility loci were also discussed in this paper.

关 键 词：水稻 粳型亲籼系 花粉不育性 复等位基因 微卫星标记 

分 类 号：S511.03[农业科学—作物学]