p5CS基因在蒙农杂种冰草植株中的表达及耐盐性研究  

Studies on the Expression of Exogenous P5CS Gene in Transgenic Wheatgrasses(Agropyron cristatum×A.desertorum cv.′Hycrest-Mengnong′)and Drought Resistance

作　　者：王桂花[1] 米福贵[2] 刘娟[2] 董淑君[2] 霍秀文[3] 杨宏雁 

机构地区：[1]内蒙古农业大学生物工程学院,内蒙古呼和浩特010018 [2]内蒙古农业大学生态环境学院,内蒙古呼和浩特010018 [3]内蒙古农业大学农学院,内蒙古呼和浩特010018 [4]呼和浩特市草原工作站,内蒙古呼和浩特010010

出　　处：《华北农学报》2007年第4期33-36,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家转基因植物与产业化研究专项资助项目(J2002-B-008)

摘　　要：为尽快培育出适宜我国干旱荒漠地区大面积推广的耐盐冰草新品种,试验以经PCR和Southern blot杂交检测的含有p5CS基因的蒙农杂种冰草植株为材料,用Northern blot检测目的基因在转化植株中的表达;并用1.5%NaCl盐溶液进行胁迫处理确定转化植株的耐盐性。结果表明:转基因冰草植株与DIG标记探针杂交呈现明显的杂交带;盐胁迫下,游离脯氨酸含量增加较快,质膜透性、丙二醛含量增加较小,SOD活性较高。说明p5CS基因能够在冰草基因组的转录水平上表达,表达植株的耐盐性明显增加。In order to breed a new salt tolerance variety which adapt to extend in drought areas in China, The p5cs gene expressed in transgenic hybrid wheatgrass （ Agropyron cristatum A. desertorum cv.＇ Hycrest-Mengnong＇）, which indentified by PCR analysis and Southern blot, was investigated with Northern blot and physiological index was determined under 1.5 % NaCl stress. The results showed that transgenic wheatgrasses and DIG marker hybridize was present obvious strap;under salt stress, Pro contents was increased quickly, cell membrane permeabilities and MDA contents were increased slowly, SOD activity was high. The results indicated that exogenous gene p5CS expressed at transcription level in transgenic plants, and salt tolerance of transgenic plants were enhanced.

关 键 词：P5CS基因 Nouthem BLOT 盐胁迫 耐盐性 

分 类 号：S543.935.3[农业科学—作物学]