高粱AFLP分子连锁图谱的构建  

Construction of an Amplified Fragment Length Polymorphism Linkage Map of Sorghum

作　　者：崔江慧[1] 薛薇[1] 段西飞[1] 王静华[1] 常金华[1] 

机构地区：[1]河北农业大学,河北保定071000

出　　处：《华北农学报》2007年第4期120-123,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金(30170498);河北省自然科学基金资助项目(2005000254)

摘　　要：利用感蚜高粱品种千三与抗蚜品种河农16杂交获得的100个F2单株作为图谱构建群体,以AFLP标记构建高粱连锁图谱。通过对192对AFLP引物组合进行筛选,共得到75对多态性引物,利用筛选出的75对引物进行选择性扩增,得到了154个AFLP多态性位点。经Map maker/EXP 3.0软件处理,构建了包含12个连锁群,93个遗传标记的连锁图谱,该图谱覆盖686 cM,平均图距为7.38 cM。A molecular genetic map for sorghum was constructed based on AFLPs （Amplified Fragment Length Polymorphism） markers using DNA samples extracted from 100 F2 population which derived from the cross between QS and HN-16. By screening 192 AFLP primer combinations, 154 polymorphic bands were obtained from 75 pairs of primers. The highly dense linkage map constructed by using Map maker 3.0 software analysis which consisted of 12 linkage groups, a total of 93 newly screened markers and covered 686 cM of sorghum genome, with the average interval of 7.38 cM.

关 键 词：高粱 连锁图谱 AFLP标记 

分 类 号：S514.01[农业科学—作物学]