大白菜线粒体DNA提取及细胞质雄性不育系RAPD分析  

Isolation of Mitochondrial DNA and RAPD Analysis with Three Cytoplasmic Male Sterilities in Chinese Cabbage

作　　者：张德双 王国臣[2] 张凤兰 王永健 方智远[3] 余阳俊 赵岫云 徐家炳 

机构地区：[1]北京蔬菜研究中心,北京100097 [2]首都师范大学,北京100037 [3]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《华北农学报》2007年第4期124-129,共6页Acta Agriculturae Boreali-Sinica

基　　金：北京市自然基金项目(6012008);北京市委组织部优秀人才项目(200503)

摘　　要：提取高质量线粒体DNA(mtDNA)是研究大白菜细胞质雄性不育系不育分子特性的前提。以大白菜绿色叶片为试验材料,应用一种省时、简便、操作步骤少的方法,快速获得3组11份大白菜的mtDNA(包括保持系、Ogu CMS、Pol CMS和CMS96细胞质雄性不育系)。试验中不需要加入DNase I酶消化核DNA,提取的mtDNA质量较好;同时,采用RAPD技术,对11份大白菜mtDNA进行多态性分析,获得了与大白菜Ogu CMS和CMS96不育系相关的差异片段10条,与大白菜Ogu CMS不育系相关的差异片段3条。初步证实:大白菜CMS96和大白菜Ogu CMS不育系既有相似性又存在差异性。Isolation mitochondrial DNA（mtDNA） is the first step for studying molecular characteristics in cytoplasmic male sterile Chinese cabbage （ Brassica campestris L. ssp. pekinensis ）. A simple and economical method was introduced in this article. Green leaves were used without adding DNase I enzyme to eliminate nuclear DNA in it. 11 mtDNAs in Chinese cabbage（including maintainers and 3 cytoplasmic male sterilities： Ogu CMS, Pol CMS and CMS96） were isolated by it. Meanwhile, through RAPD technique, 10 special fragments linked to Ogu CMS and CMS96 were found.3 special fragments linked to Ogu CMS were found. It primarily proved that the male molecular characteristics of Ogu CMS and CMS96 in Chinese cabbage had similarities as well as differences

关 键 词：大白菜 细胞质雄性不育 MTDNA 提取 RAPD 

分 类 号：S634.1[农业科学—蔬菜学]