谷子品种遗传差异的RAPD标记分析  

Genetic Variation Among Varieties of Foxtail Millet(Setaria italica Beauv.) Based on RAPD Markers

作　　者：杨延兵[1] 管延安[1] 张华文[1] 徐平平[2] 张文兰[1] 陈利容[1] 秦岭[1] 

机构地区：[1]山东省农业科学院作物研究所,山东济南250100 [2]陕西师范大学,陕西西安710062

出　　处：《华北农学报》2007年第4期134-136,共3页Acta Agriculturae Boreali-Sinica

基　　金：山东省农业科学院青年科研基金项目资助(2005YQ051);国家科技支撑计划资助(2006BAD02B02)

摘　　要：利用RAPD标记对来自春、夏谷区不同育种单位的23份谷子品种进行了多态性和聚类分析。结果表明,23份品种的RAPD标记的多态性较低,13个引物共扩增出56条多态性带,平均每个引物扩增多态性带为4.31条,引物S30和S45都有7条多态性带,能够鉴别的品种最多为13个。聚类分析表明,当遗传相似性系数为0.75时,23个品种可分为4类,不同生态区的品种可以归为一类,而同一生态区的品种也可以归为不同的类,基于RAPD的遗传相似性和品种来源地没有必然的一致性。Genetic variation of 23 varieties of foxtail millet collected from spring millet region and summer millet regions was studied. The results showed that 23 varieties had certain degree of genetic variation in molecular level, but the degree of variation was not significant. 13 primers yielded 56 polymorphic bands from 23 varieties and the average of polymorphic bands was 4.31 yielded by each primer. The primers S30 and S45 yielded 7 poiymorphic bands, respectively, and which could be used to distinguish 13 varieties. The cluster analysis of RAPD makers indicated that the cluster type of genetic variation of varieties was not consistent with ecological type. Varieties from different regions could be clustered in the same type, and varieties from same regions might be clustered in different types.

关 键 词：谷子 遗传差异 RAPD标记 

分 类 号：S515.01[农业科学—作物学]