FMDV OA/58病毒株VP1蛋白结构构建与B细胞抗原表位的预测  

Construction of VP1 Protein and Prediction of B Cell Epitopes in VP1 from a Foot-and-mouth Disease Virus Strain OA/58

作　　者：周建华[1] 丛国正[1] 高闪电[1] 常惠芸[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室国家口蹄疫参考实验室,甘肃兰州730046

出　　处：《华北农学报》2007年第4期176-179,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家重点基础研究发展计划国家"973"项目(2005CB523201)

摘　　要：以口蹄疫病毒株OA/58 RNA为模板,反转录并扩增目的cDNA,然后与pGEM-T easy载体连接并转化JM109菌株,提取的重组质粒用凝胶电泳、PCR和EcoR I酶切法鉴定。运用同源模建得到OA/58 VP1蛋白3D结构,并结合理化性质、亲水性、可塑性和免疫原性进行分析,预测OA/58 VP1的抗原表位。结果OA/58 VP1存在多个潜在的抗原表位位点,可能的蛋白质抗原表位区域:2～11,15～35,38～50,77～88,90～107,121～125,131～135,140～149,154～163,169～175,178～189,197～213。应用同源模建得到的OA/58 VP1蛋白3D模型来预测其B细胞表位,为进一步研究OA/58 VP1功能,构建突变体和选择表达新型OA/58 VP1蛋白分子提供有参考价值的信息。Foot-and-mouth disease virus strain OA/58 RNAs were used as templates for RT-PCR. The amplified cDNA products were cloned into pGEM-T easy vectors and transformed into JM109. The recombinant plasmids were identified by electrophoresis,PCR,and EcoR I cleavage. By means of homology modeling the FMDV strain OA/58 VP1 protein, the 3D mold was obtained. In order to find out B cell epitopes of OA/58 VP1, several methods were analyzed including physical and chemical characters, hydrophilicity, antigenicity. Many distinct antigenic epitopes in FMDV OA/58 VP1 were identified by computation： 2 - 11,15 - 35,38 - 50,77 - 88,90 - 107,121 - 125,131 - 135,140 - 149,154 - 163,169- 175,178- 189,197- 213. Application of homology molding OA/58 VP1 to predict B cell epitopes is reasonable. This method offers reasonable information for researching function of OA/58 VP1, constructing its variant body and selecting new expression forms of OA/58 VP1.

关 键 词：口蹄疫病毒 VP1蛋白 B细胞抗原表位 

分 类 号：S852.65[农业科学—基础兽医学]