甲苯降解菌降解特性及代谢途径初步研究  

Analysis of Biodegradation Characteristics and Metabolic Pathway of Toluene-degrading Bacteria

作　　者：饶佳家[1] 霍丹群[2] 

机构地区：[1]重庆工学院生物工程学院,重庆400050 [2]重庆大学生物工程学院,重庆400044

出　　处：《西南师范大学学报（自然科学版）》2007年第4期64-67,共4页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家自然科学基金(No.50006015)

摘　　要：从污泥中分离得到1株能以甲苯为唯一碳源和能源生长的细菌菌株,经过生理生化实验初步鉴定为假单胞菌属.温度在20～40℃之间、pH7、甲苯浓度在316 mg/L以下,菌株降解甲苯的效率保持80%～100%之间,甲苯浓度大于316 mg/L,出现抑制现象.甲苯降解途径是通过甲苯双加氧酶形成顺甲苯二氢二醇,再经邻苯二酚2,3双加氧酶开环降解.A bacterial strain isolated from waste sludge was identified as Pesudomonas putida according to morphological, physiological experiment. It can use toluene as sole carbon and energy sources. Under experimental conditions, the strain degraded 80%-100%of the added toluene at temperature 20-40℃ and pH for 7. The toluene concentrations under 316mg/L can not significantly affect its removal efficiency. The biodegradation pathway of toluene has formed cis-toluene dihydrodiol by toluene dioxygenase.

关 键 词：甲苯 降解 甲苯双加氧酶 代谢途径 

分 类 号：X511[环境科学与工程—环境工程]