大孔树脂富集纯化藏药独一味总环烯醚萜苷  

Research on isolation and purification of total iridoid glycosides from herbal Lamiophlomis rotata

作　　者：李茂星[1] 贾正平[1] 胡之德[2] 张汝学[1] 任晓玲[1] 王娟[1] 胡静[1] 

机构地区：[1]兰州军区兰州总医院药材科,甘肃兰州730050 [2]兰州大学化学化工学院,甘肃兰州730000

出　　处：《中国中药杂志》2007年第17期1743-1747,共5页China Journal of Chinese Materia Medica

基　　金：全军中医药研发推广项目(2006032001);甘肃省科技攻关项目(2GS054-A43-014-14)

摘　　要：目的:进行藏药独一味止血活性成分总环烯醚萜苷的富集纯化。方法:以一阶导数分光光度法检测,以总环烯醚萜苷含量为跟踪指标,比较10种不同厂家、不同型号的大孔树脂对独一味水提取物中环烯醚萜苷类成分的吸附与解吸附条件,优选最佳的纯化工艺。结果:富集后总环烯醚萜苷的含量达到67.53%。结论:XDA-1型大孔吸附树脂对独一味水提取物中环烯醚萜苷类成分有较好的吸附能力,50%乙醇溶液解吸附快速有效。树脂重复利用度高。Objective： Enriching the hemostatic active part total iridoid glycosides from the aqueous extract of herbal Lamiophlomis rotata. Method： First derivative spectrophotometry was used as the detection method and the content of total iridoid glycosides was used as tracking target. The abilities of absorption and anti-absorption of ten different types of macroreticular resin were compared, after being used to absorb the iridoid glycosides from aqueous extract of herbal L. rotata. The best purification process was optimized. Result： With the optimized purification process, the content of total iridoid glycosides was 67.53 %. Conclusion： XDA-1 macroreticular resin has good ability of absorption and anti-absorption for iridoid glycosides and 50% ethanol was the best solvent for anti-absorption. The maeroreticular resin could be reused.

关 键 词：独一味 总环烯醚萜苷 大孔树脂 工艺 

分 类 号：R29[医药卫生—民族医学]