猪瘟病毒E2基因的克隆及其初步鉴定  

Cloning of Swine Fever Virus E2 Gene and Its Initial Identification

作　　者：杨文超[1] 谢金文[1] 余为一[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036

出　　处：《安徽农业科学》2007年第25期7841-7841,共1页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为了研究猪瘟病毒E2蛋白的抗原性质和进一步筛选保护性抗原表位。[方法]克隆猪瘟病毒E2蛋白的部分基因片段,通过自行设计的一对引物,应用RT-PCR从猪瘟兔化弱毒株细胞培养物,扩增出一DNA片段,并对其进行鉴定。[结果]经初步鉴定,该片段大小约703 bp,核苷酸序列测定结果表明该片段为E2蛋白基因。[结论]该研究为筛选CSFV抗原奠定了基础。　In order to study the characters of swine fever virus（SFV） E2 antigen and select its protection epitope,a gene fragment was amplified with a pair of designed primers from the cultured cells infected SFV by RT-PCR.The results of initial identification indicated that this fragment was about 703 bp and its nucleotide sequence was identity with SFV-E2 gene reported in Gene Bank.The study laid a foundation of screening CSFV antigen.

关 键 词：猪瘟病毒 E2基因 克隆 

分 类 号：Q785[生物学—分子生物学]