小鼠恒定链基因的克隆及鉴定  

Cloning and Identifying of Mouse Invariant Chain Gene and Its Identification in Prokaryotic Cells

作　　者：董林[1] 余为一[1] 许发芝[1] 刘岗[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036

出　　处：《安徽农业科学》2007年第25期7856-7856,7861,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为了比较小鼠Ii链在机体免疫应答中的作用。[方法]通过自行设计的一对引物,应用RT-PCR方法,从小鼠脾脏中扩增出恒定链基因片段。[结果]经酶切和初步测序,结果表明该基因片段长度为700 bp,其DNA序列与Genbank登录的鼠Ii同源性高达99.2%。[结论]该研究为进一步研究小鼠Ii基因结构和免疫学功能提供了一定基础。　The objective of the study is to compare the functions of mouse invariant chain in body immune response.A total RNA was obtained from mouse spleen cells.Then the gene of invariant chain（Ii） of mouse was amplified from the RNA by reverse transcription（RT）-PCR with a pair of designed primers.The fragment was identified by a restriction endonuclease.The results indicated that the length of this cloned fragment was 700bp and its homology with the sequence of the mouse Ii in Gene Bank is up to 99.2%.

关 键 词：小鼠 II基因 克隆 

分 类 号：Q785[生物学—分子生物学]