毛唇独蒜兰的离体快速繁殖研究  

Study on tissue culture of pleione hookeriana

作　　者：于晓娟[1] 纳海燕[1] 胡晓丽[1] 魏兴强 范昆 刘方媛[3] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]云南英茂生物农业技术公司,安宁650301 [3]中科院昆明植物研究所,昆明650204

出　　处：《四川大学学报（自然科学版）》2007年第4期891-894,共4页Journal of Sichuan University(Natural Science Edition)

摘　　要：以毛唇独蒜兰的原球茎块作为外植体进行快速繁殖的研究,结果表明:毛唇独蒜兰(Pleione hookeriana)在1/2MS+6-BA 1 mg/L下丛生芽的出芽率为80%,而在TH+NAA 0.2mg/L+6-BA 1 mg/L下丛生芽的出芽率93%,并且增殖倍数比1/2MS多.在MS+土豆50 g/L培养基有利于毛唇独蒜兰原球茎增重.诱导丛生芽的原球茎切块大小不小于0.5 cm×0.5 cm×0.5 cm.The protocorms of Pleione hookeriana in vitro were used as explants for the rapid propagation. The results were stated as follows： the multiple shoots rate of protocorems of P. hookeriana was 80% in 1/2 MS medium＋ 1 mg/L 6-BA, however that of explants was 93% in TH medium ＋ 0.2 mg/L NAA ＋ 1 mg/L 6-BA.TH medium is better than 1/2 MS medium on proliferation. MS ＋ 50 g/L potato was optimal for the weight of protocorms of P. hookeriana. The explants producing multiple shoots should be bigger than 0.5 cm ×0.5 cm×0.5 cm.

关 键 词：毛唇独蒜兰 丛生芽诱导 原球茎 

分 类 号：Q943[生物学—植物学]