巴西红苋的组培快繁和扦插繁殖研究  

Vegetative propagation and cuttage of Iresine herbstii in vitro

作　　者：钟家骥[1] 周浩[1] 王莹 纳海燕[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]成都市园林科研所,成都610083

出　　处：《四川大学学报（自然科学版）》2007年第4期907-911,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：成都市建委应用研究基金(2004722)

摘　　要：以红苋茎段和嫩叶作为外植体进行组织培养获得丛生芽,再通过增殖、生根等阶段得到完整试管苗.实验表明：外植体取材季节以4-7月较好;最适增殖培养基为MS＋BA 2.0mg/L＋NAA 0.2 mg/L＋30 g/L蔗糖,其增殖倍数达到4.0以上;而生根培养基以1/2MS＋NAA 0.5 mg/L＋20 g/L蔗糖较好.扦插繁殖试验表明,在草炭＋蛭石＋珍珠岩的混合土壤基质上获得最高的枝条扦插成活率和平均根数.The stems and tender leaves of Iresine herbstii were used as explants for tissue culture to obtain cluster buds, which then grew to be the complete test-tube plantlets through the multiplication phase and rooting phase. The experiments indicated that the best time for getting explants were from April to July, the optimum multiplication medium was MS ＋ BA 2.0 mg/L ＋ NAA 0.2 mg/L ＋ 30 g/L sucrose ＋ 1.0 % agar, whose multiplication rate reached over 4.0, and the medium of 1/2MS ＋ NAA 0.5 mg/L＋ 20 g/L sucrose＋ 1.0 % agar was better for root media than others. The cutting propagation for Iresine herstii showed that the survival rate of shoot cutting and the average root amount were the highest on the mixing soil base of grass charcoal, vermiculite and pearlite.

关 键 词：巴西红苋 组培快繁 扦插 生根 

分 类 号：Q943[生物学—植物学]