关节内注射复方倍他米松对兔佐剂性关节炎的效果  

Mechanism of intra-articular diprospan on adjuvant-induced arthritis in rabbits

作　　者：李志华[1] 柳顺锁[1] 宛春甫[2] 张雷波 孟庆云[1] 赵东[1] 宋万军[1] 

机构地区：[1]河北医科大学第三医院麻醉科,石家庄市050051 [2]河北医科大学第四医院疼痛康复科 [3]江苏省无锡市第二医院麻醉科

出　　处：《中华麻醉学杂志》2007年第7期607-609,共3页Chinese Journal of Anesthesiology

摘　　要：目的评价关节内注射复方倍他米松对兔佐剂性关节炎的效果。方法日本大耳白兔，雌雄各半，体重2～3kg，6～8月龄。采用皮内注射卵清蛋白＋非完全弗氏佐剂的方法制备佐剂性关节炎模型。佐剂性关节炎模型制备成功的48只免随机分为2组（n=24），C组双膝关节内各注射生理盐水1ml；D组注射复方倍他米松0．5mg／kg。每组各随机取6只兔，持续观察膝关节外观，分别于治疗前、治疗后2周测量髌骨上缘处双膝周径，并测定膝关节灌洗液中一氧化氮（NO）浓度，治疗后2周处死兔，打开膝关节，观察关节腔内改变，取髌上囊处滑膜，光镜下观察病理学改变。2组分别于治疗后1、2、3d各随机处死6只兔，取髌上囊滑膜，观察细胞凋亡情况。结果与c组比较，治疗后D组关节滑膜炎性反应较轻，NO浓度降低（P〈0．01）；治疗后2、3d滑膜细胞凋亡率升高（P〈0．05或0．01）。结论膝关节内注射复方倍他米松0．5mg／kg对免佐剂性关节炎有抗炎作用，可能与抑制NO的合成及促进滑膜细胞凋亡有关。Objective To investigate the effect of intra-articular diprospan on the adjuvant-induced arthritis in rabbits and the mechanism. Methods Forty-eight Japanese white rabbits of both sexes weighing 2-3 kg were used in this study. The adjuivant arthritis was induced by intradermal injection of Freund＇s adjuvant 0.25 ml and ovalbumia 5 mg in 0.25 ml once a week for 2 weeks followed by injection of ovalbumin 0.5 ml （10 mg/kg ） into bilateral knee joints. The typical signs of arthritis developed one week later （ swollen joints, congestion and hyperthermia of the skin over the knee joints） , The animals were randomly divided into 2 groups （ n = 24 each） ： C control group received intra-articular injection of normal saline 1 ml; D deprospan group received diprospan 0.5 mg/kg in 1.0 ml. Before intra-articular injection the animals were anesthetized with intramuscular ketamine 50 mg/ kg. The animals were sacrificed at day 14 after injection. The specimens were obtained for microscopic and histological assessment, The NO concentration in douching fluid in the knee joints was determined, On the 1 st, 2nd and 3rd day after injection the apoptosis in synoviocytes was assessed. Results （ 1 ） Diprospan produced a significantly better results in group D than normal saline in group C in terms of microscopic findings. （2） NO concentration in the douching fluid was significantly lower in group D than in group C. （3） The apoptosis rate of synoviocytes was significantly higher in group D than in group C. Conclusion Intra-articular diprospan 0.5 mg/kg has anti-inflammatory effect on adjuvant-induced arthritis in rabbits. The inhibition of the synthesis of NO and acceleration of apoptosis in synoviocytes are involved in the underlying mechanism.

关 键 词：倍他米松 关节炎 实验性 注射 关节内 

分 类 号：R686[医药卫生—骨科学]