反相高效液相色谱法同时测定人血浆中对乙酰氨基酚/曲马朵及其代谢物的含量  

SIMULTANEOUS DETERMINATION OF PARACETAMOL/TRAMADOL AND ITS METABOLITE IN HUMAN PLASMA BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：周芬[1] 孙莉[1] 刘丽京[1] 邓艳萍[1] 

机构地区：[1]北京大学中国药物依赖性研究所,北京100083

出　　处：《中国药物依赖性杂志》2007年第4期271-275,共5页Chinese Journal of Drug Dependence

摘　　要：目的:建立一种用高效液相色谱法(HPLC)同时测定人血浆中对乙酰氨基酚/曲马朵及其代谢物氧去甲基曲马朵(M1)浓度的方法。方法:以间乙酰氨基酚为内标,血浆样品经碱化后,用乙酸乙酯提取。HPLC-UV测定采用DiamonsilTM C18色谱柱(250mm×4.6mm),流动相为0.01mol·L-1磷酸二氢钾缓冲液(含三乙胺0.005mol·L-1,磷酸调pH3.0)-乙腈(79:21),流速:1.0ml·min-1,紫外检测波长:218nm。结果:本实验条件下各物质分离良好,对乙酰氨基酚在0.2-21.6μg·ml-1范围呈线性,曲马朵及其代谢物M1分别在10-720和5-160ng·ml-1范围呈线性。对乙酰氨基酚的最低检测限为0.2μg·ml-1,曲马朵和M1的最低检测限分别为10和5ng·ml-1。三种物质的方法回收率均在80%-120%范围内,提取回收率均在75%以上。结论:本法简单、快速,可用于人血浆中对乙酰氨基酚/曲马朵及M1的同时检测。Objective ：To establish a reversed - phase high performance liquid chromatography（HPLE） method for the determination of paracetamol/tramadol and its metabolite O-desmethyltramadol（ M1 ）in human plasma. Methods： M -acetaminphen was used as an internal standard, and alkalinized samples of plasma were extracted with ethyl acetate. Chromatographic separation was achieved with a Diamonsil^TM C18 analytical column（250 mm ×4. 6 mm）. The mobile phase was 0. 01 mol·L^-1potassium dihydrogen phosphate （0. 005 mol·L^-1 triethylamine,pH 3.0 adjusted by phosphoric acid） - acetonitrile（79：21 ）, and flow rate was 1.0 ml ·min^-1. The drug concentrations were measured by UV at 218 nm. Results ：The calibration curves were linear in the concentration range of 0. 2 -21.6 tzg .m1-1, 10 -720 ng .m1-1 and 5 - 160 ng .m1-1 for paracetamol,tramadol and M1, respectively. The limit of quantition（LOQ） was 0. 2μg·ml^-1for paracetamol, 10 ng ·ml^-1 for tramadol and 5 ng·ml^-1for M1. The recovery of the three samples was within 80% -120%, the absolute recovery was above 75%. Conclusion：This method is simple and rapid, especially for quantitative analysis of paracetamol/tramadol in human plasma simultaneously.

关 键 词：高效液相色谱法 对乙酰氨基酚 曲马朵 氧去甲基曲马朵 

分 类 号：R927.2[医药卫生—药学]