4种南极冰藻中抗紫外辐射活性化合物类菌胞素类氨基酸(MAAs)的初步研究  

Preliminary study on the antiultraviolet irradiation active substances of mycosporine-like amino acids from four kinds of Antarctic ice microalgae

作　　者：杜宁[1] 高天翔[1] 缪锦来[2] 张波涛[1] 王守强[2] 王斌[2] 

机构地区：[1]中国海洋大学水产学院,山东青岛266003 [2]国家海洋局第一海洋研究所,山东青岛266061

出　　处：《中国海洋药物》2007年第4期5-10,共6页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金资助项目(No.4020602240506005)

摘　　要：目的研究和发现极端环境下南极冰藻中抗紫外辐射活性化合物。方法对4种生长较快的南极冰藻进行低温光照培养,同时采用UV-A和UV-B辐照胁迫,提取分离抗紫外辐射活性化合物;根据其理化性质、仪器分析数据初步确定各化合物的分子结构。并通过日光辐照活性组分,观察其降解速度,确定其抗紫外辐射活性。结果从南极绿藻Chlorophyceae L-4,硅藻Diatom HF-9中分别得到5种、7种活性化合物,其中6种属于类菌胞素类氨基酸(MAAs)类物质,并具有较强的抗紫外辐射活性。结论MAAs类物质在南极冰藻抗强紫外辐射过程中扮演重要角色。Objective To study on bioactive chemical compounds against ultraviolet irradiation in antarctic ice microalgae. Methods Four kinds of Antarctic ice microalgae were cultured under the conditions of low temperature, illumination, UV-A and UV-B irradiation . The chemical compounds were extracted by methanol and analyzed by spectrophotometer and HPLC/MS. The molecular structures were elucidated according to these data. The chemical compounds were irradated by sunshine to observe the speed of degradation and to estimate the bioactivity. Results There were five active compounds in Chlorophyceae L-4 and 7 active compounds in Diatom HF-9, in which 6 compounds belong to MAAs（mycosporine-like amino acids） and were of more bioactivity against ultraviolet irradiation. Conclusion MAAs from of Antarctic ice microalgae play an important role in resisting ultraviolet irradiation.

关 键 词：南极冰藻 紫外辐射 类菌胞素类氨基酸 

分 类 号：R931.711[医药卫生—生药学] R965[医药卫生—药学]