山羊FSHR基因5′端侧翼序列多态性对产羔性状的影响  

Effect of the 5′-flanking region of goat follile-stimulating hormone receptor gene on yean trait

作　　者：姬云涛[1] 曹斌云[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2007年第9期1-4,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"863"计划项目(2002AA242051)

摘　　要：为了研究山羊FSHR基因对产羔性状的影响,用RFLP-PCR法对多胎和单胎布尔山羊、关中奶山羊和萨能奶山羊母羊FSHR基因5′端侧翼序列进行了多态性检测。结果发现,FSHR基因5′端侧翼序列经PstⅠ、HaeⅢ、SinⅠ和TaqⅠ酶切,在多胎母羊和单胎母羊群体中均未表现出多态性;不同山羊群体FSHR基因5′端侧翼序列中各存在一些碱基的差异,但这些差异均未影响FSHR基因的转录和山羊的产羔性状。说明所检测的FSHR基因5′端侧翼序列这些酶切位点与山羊的产羔性状之间没有遗传连锁关系。Boer goat, Guanzhong goat and Xinongsaneng goat with multiplets and monovular traits were used to study the effect of follile-stimulating hormone receptor gene on yeant rait. Polymorphism of the 5＇-flanking region of goat follile-stimulating hormone receptor gene was analyzed by PCR-RFLP as the candidate gene. The result showed there wasn＇t polymorphism in goat. Difference of several bases were found by sequening, but the transcription of follile-stimulating hormone receptor gene and yean trait of goat were not affected,which showed that there was not the genetic linkage relationship between the four sites of 5＇-flanking region of goat FSHR gene and its yean trait.

关 键 词：山羊 FSHR基因 产羔性状 RFLP-PCR 

分 类 号：S827[农业科学—畜牧学]