丙戊酸钠对人乳腺癌细胞系MCF-7增殖的抑制作用及其量效关系研究  

Inhibiting Effect of VPA on the Proliferation of MCF-7 and the Dose-effect Relationship

作　　者：顾卫平[1] 李宏建[2] 谷大建[2] 时昌文[2] 

机构地区：[1]山东大学药学院,济南市250012 [2]山东省千佛山医院,济南市250014

出　　处：《中国药房》2007年第25期1937-1939,共3页China Pharmacy

摘　　要：目的:探讨丙戊酸钠(VPA)对乳腺癌细胞系MCF-7的增殖抑制作用及其量效关系。方法:MCF-7细胞用0.75～4.0mmol·L-1VPA处理48h,同时设不加药对照组。MTT法分析细胞生长抑制、AnnexinⅤ/碘化丙啶双染法检测细胞凋亡、碘化丙啶法观察细胞增殖周期的变化。结果:与对照组比较,VPA组细胞生长抑制率由17%～54%递增,细胞凋亡率中位数分别为8.32%、9.6%、11.8%、13.7%、16.6%,与对照组比较均有不同程度的增加;细胞增殖周期分析可见对照组顺序出现G1、S、M期,而VPA组随药物浓度的升高而出现逐渐递增的G1期阻滞。结论:VPA可明显抑制MCF-7的生长,诱导凋亡和细胞周期G1期阻滞作用,且呈剂量依赖趋势,在乳腺癌治疗方面有重要价值。OBJECTIVE： To observe the inhabiting effect of valproate acid sodium （VPA） on MCF- 7, a human breast carcinoma cell line, and to study its dose - effect relationship. METHODS： MCF- 7 cells were treated with 0.75-4.0mmol · L^-1 VPA for 48 hours, a control group was established and which was not treated with VPA. The inhibition rate was studied by MTT assay; the apoptosis was detected by Annexin V/PI assays and the cell cycle was analyzed by PI assay. RESULTS： As compared with control group, the inhibition rate of MCF- 7 cells treated with VPA increased from 17% to 54%, the median apoptosis rates were 8.32%, 9.6%, 11.8%, 13.7%, and 16.6%, respectively, higher than in control group. G1, S, and phases appeared in order in cell generation cycle in the control group, while arrest of MCF- 7 appeared in G1 phase dose- dependently in VPA group. CONCLUSION： VPA can significantly inhibit the growth of MCF 7, and induce apoptosis and cell cycle arrest in G1 phase in a dose-dependent manner, and which has significant value in the treatment of breast cancers.

关 键 词：乳腺癌细胞系MCF-7 丙戊酸钠 生长抑制 凋亡 细胞周期 量效关系 

分 类 号：R965[医药卫生—药理学] R971.6[医药卫生—药学]