活血通络口服液中黄芪甲苷的含量测定  

Quantitative Determination of Astragaloside IV in Huoxuetongluo Oral Liquid by HPLC-ELSD

作　　者：王秀萍[1] 常军民[1] 曾平[2] 

机构地区：[1]新疆医科大学,新疆乌鲁木齐830000 [2]兰州军区乌鲁木齐总医院药剂科,新疆乌鲁木齐830000

出　　处：《中国药业》2007年第17期12-13,共2页China Pharmaceuticals

摘　　要：目的建立活血通络口服液中黄芪甲苷的含量测定方法。方法采用高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定,色谱柱为Discoverry C18柱(250mm×4.6mm,5μm),流动相为甲醇-水(100∶20),流速为1.0mL/min,漂移管温度为105℃。结果以峰面积对数值(Y=lgA)为纵坐标、进样量对数值(X=lgC)为横坐标的线性回归方程为Y=1.8218X+4.7306,r=0.9988;进样量线性范围是0.551～5.51μg,平均加样回收率为97.7%,RSD为0.45%。结论HPLC-ELSD法准确度高、干扰少、简便快捷,可用于活血通络口服液的质量控制。Objective To establish a method of determining the content of astragaloside Ⅳ in Huoxuetongluo oral liquid. Methods To determine the content of astragaloside in Huoxuetongluo oral liquid by HPLC-ELSD. The chromatography C18 （250 mm ×4. 6 mm, 5 μm） column was as the stationary phase, methanol-water （100：20） as the mobile phase. Temperature of drift tube was 105℃. Results The average recovery rate of astragaloside Ⅳ was 97.7%, RSD =0. 45%. Conclusion This method is sensitive,accurate,and simple with little interference for the determination of astragaloside Ⅳ content. The method can be used for controlling the quality of Huoxuetongluo oral liquid.

关 键 词：活血通络口服液 黄芪甲苷 高效液相色谱-蒸发光散射检测法 

分 类 号：R286.0[医药卫生—中药学] R284.1[医药卫生—中医学]