薄层色谱扫描法测定蛇胆川贝液中牛磺胆酸含量  

Determination of Taurocholic Acid in Shedanchuanbei Solution by TLCS

作　　者：黎明[1] 

机构地区：[1]广西医科大学制药厂,广西南宁530021

出　　处：《中国药业》2007年第17期23-23,共1页China Pharmaceuticals

摘　　要：目的建立以薄层扫描法测定蛇胆川贝液中牛磺胆酸含量的方法。方法固定相为硅胶G加0.5%羧甲基纤维素钠(1∶2.5)所制备的薄层板,显色剂为10%磷钼酸乙醇溶液,展开剂为氯仿-异丙醇-冰醋酸-水(25∶30∶4∶1),检测波长为623nm。扫描方式为单波长反射法锯齿扫描,光源钨灯,线性参数SX=3,振幅为10,背景校正。结果牛磺胆酸的平均回收率为100.36%,RSD为1.89%,线性范围是0.25～2.5mg/mL(r=0.9919)。结论薄层扫描法测定蛇胆川贝液中牛磺胆酸的含量,准确度高,重现性好,适合工业生产中的快速检验。Objective To determine the content of the taurocholic acid in Shedanchuanbei solution by TLCS. Methods To use the TLCS method for detecting the content of the taurocholie acid. Results The average recovery rate was 100. 36% （RSD = 1.89% ）. Within the range of 0. 25-2.5 mg/mL, there was a linear relationship with a value for r =0. 991 9. Conclusion This method is accurate and repeatable.

关 键 词：牛磺胆酸 薄层扫描法 含量测定 

分 类 号：R286.0[医药卫生—中药学] R284.1[医药卫生—中医学]