易喘平胶囊对哮喘模型小鼠气道高反应性的抑制作用及机制  

Inhibiting effect and mechanism of Yichuanping capsule (易喘平胶囊) on the airway hyper-responsiveness in asthmatic mouse

作　　者：宋立强[1] 马战平[2] 李妍[3] 张乐宁[4] 高放[4] 程九华[4] 

机构地区：[1]第四军医大学西京医院呼吸内科,陕西西安710033 [2]陕西中医药研究院 [3]第四军医大学西京医院心脏内科 [4]第四军医大学空医系

出　　处：《中国中西医结合急救杂志》2007年第5期264-266,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金资助课题(30400197);陕西省中医药管理局资助课题(0386)

摘　　要：目的:观察补肾活血法中药易喘平胶囊对哮喘气道高反应性的抑制作用及其机制。方法:将50只雄性BALB/c小鼠随机分为正常对照组、哮喘模型组、地塞米松组(0.4mg·kg-1·d-1)以及易喘平胶囊高剂量(20mg·kg-1·d-1)和低剂量组(5mg·kg-1·d-1)。用卵白蛋白致敏法制备哮喘模型,检测各组小鼠不同乙酰甲胆碱(mACh)浓度时气道压力峰值-时间指数(APTI)的差异,并比较各组小鼠支气管肺泡灌洗液(BALF)中内皮素-1(ET-1)和一氧化氮(NO)的含量。结果:哮喘模型小鼠不同mACh浓度时的平均APTI值和BALF中ET-1和NO含量均明显高于正常对照组(P均<0.05)。与哮喘模型组比较,高剂量、低剂量易喘平胶囊组和地塞米松组的APTI值以及BALF中ET-1和NO含量均明显下降(P均<0.05);高剂量易喘平胶囊组表现出比低剂量组更强的抑制作用,呈现剂量依赖特性;高剂量易喘平胶囊组与地塞米松组的APTI值以及BALF中ET-1和NO含量比较差异无显著性(P均>0.05)。结论:易喘平胶囊对哮喘气道高反应性具有一定的抑制作用,降低气道上皮细胞分泌ET-1和NO是这种作用发挥的机制之一。Objective： To explore the inhibiting effect and mechanism of Yichuanping capsule （易喘平胶囊） on the airway hyper- responsiveness （AHR） in asthmatic mice. Methods： Fifty male BALB/c mice were randomly divided into normal control group, asthma group, dexamethasone group （0.4 mg·kg^-1·d^-1, high dose of Yichuanping group （HDY, 20 mg·kg^-1·d^-1） and low dose of Yichuanping group （LDY, 5 mg·kg^-1·d^-1）. The model of asthma was induced by ovalbumin sensitization. The airway press - time index （APTI） of different methacholine （mACh） concentration, the content of endothlin - 1 （ET - 1） and nitric oxide （NO） in bronchoalveolar lavage fluid （BALF） in all groups were checked. Results： The APTI of mACh concentration and the contents of ET - 1 and NO in BALF in asthma group were higher than those in normal control group （all P〈0.05）. Compared with asthma group, the APTI and the contents of ET - 1 and NO in BALF in HDY group, LDY group and dexamethasone group were reduced remarkably （all P〈0.05）. The inhibiting effect for AHR in HDY group was stronger than that in LDY group, and the character of dose - dependence could be observed. There was no obvious difference in APTI, contents of ET - 1 and NO in BALF between HDY group and dexamethasone group （all P〉0.05）. Conclusion： It can be demonstrated that Yichuanping capsule has the inhibiting effect on AHR in asthma, and one of mechanisms is refraining the production of ET - 1 and NO by airway epithelium.

关 键 词：易喘平胶囊 哮喘 气道高反应性 内皮素-1 一氧化氮 

分 类 号：R285.5[医药卫生—中药学]