Na^＋/Ca^2＋交换抑制剂对慢性阻塞性肺疾病患者肺泡巨噬细胞功能的影响  

Effect of Na^＋/Ca^2＋ exchange blocker, Benzamil, on alveolar macrophage function in patients with chronic obstructive pulmonary disease

作　　者：李旭[1] 王立祥[1] 张健鹏[1] 谷鸥翔 宋满景[3] 

机构地区：[1]武警总医院医学急救中心,北京100039 [2]武警部队药品仪器检验所 [3]山西医科大学第二医院

出　　处：《中国危重病急救医学》2007年第9期522-524,共3页Chinese Critical Care Medicine

摘　　要：目的观察Na^＋/Ca^2＋交换抑制剂Benzamil对慢性阻塞性肺疾病（COPD）患者肺泡巨噬细胞（AM）胞浆中游离钙离子（〔Ca^2＋〕i）浓度及其对生成肿瘤坏死因子-α（TNF-α）、丙二醛（MDA）的影响。方法采用支气管肺泡灌洗、细胞培养和荧光指示剂的方法,测定36例COPD稳定期患者及36例健康体检者AM内〔Ca^2＋〕i浓度及其生成的TNF-α和MDA含量。结果1COPD组患者AM内〔Ca^2＋〕i〔（68.26±7.24）nmol/L〕、TNF-α〔（5.74±0.42）ng/L〕和MDA〔（3.77±0.61）μg/L〕水平均较健康对照组〔分别为（60.61±6.26）nmol/L、（2.06±0.20）ng/L、（1.91±0.19）μg/L〕明显增高（P均〈0.01）;2给予缺氧后,COPD组AM内〔Ca^2＋〕i浓度〔（168.34±17.58）nmol/L〕、TNF-α〔（9.67±1.01）ng/L〕和MDA〔（11.21±1.01）μg/L〕水平均较刺激前增高（P均〈0.01）;3先加Benzamil孵育AM再缺氧,可使胞浆内〔Ca^2＋〕i浓度〔（129.21±14.33）nmol/L〕、TNF-α〔（6.78±0.52）ng/L〕和MDA〔（8.47±0.79）μg/L〕水平均较单纯缺氧时明显减少（P均〈0.01）。结论Benzamil可抑制由缺氧引起AM内〔Ca^2＋〕i浓度增高及其产生的TNF-α、MDA含量;提示通过调节AM激活可抑制TNF-α、MDA的分泌。Objective To observe the effect of Benzamil on cytosolic free calcium ions （[Ca^2＋]i） concentration, tumor necrosis factor-α （TNF -α） and malondialdehyde （MDA） in alveolar macrophage （AM） in patiens with chronic obstructive pulmonary disease （COPD）. Methods Alveolar macrophages were collected from bronchoalveolar lavage fluid,and they were cultured and [Ca^2＋]i were determined by calcium fluorescent with indicator Fura - 2/AM. The effects of Benzamil on the increase in cytosolic [Ca^2＋]i and on secretion of TNF -α, MDA were investigated in 36 patients with COPD and 36 healthy volunteers. Results （1） [Ca^2＋]i concentration （68.26±7.24） nmol/L, TNF - α （5.74±0.42） ng/L and MDA （3.77±0.61） μg/L were found in COPD patients, and they were significantly increased, as compared with the control group, in whom the contents were [Ca^2＋]i concentration （60. 61±6.26） nmol/L, TNF - α （2.06± 0. 20） μg/L, MDA （1.91±0.19） ng/L （all P〈0.01）. （2）The following data namely [Ca^2＋]i concentration （168.34±17.58） nmol/L, TNF -α （9.67±1.01） ng/L and MDA （11.21±1.01） μg/L were obtained after anoxia in COPD patients （all P〈0.01）. （3）After incubation with Benzamil, and then subjected the cells to anoxia, the following data were obtained ： [Ca^2＋ ]i concentration （129.21± 14.33） nmol/L, TNF - α content （6.78±0.52） ng/L and MDA （8.47±0.79） μg/L and they were all lower than only anoxia was applied （all P〈0. 01）. Conclusion Benzamil not only downregulates alveolar macrophages activity but also suppresses the generation of the TNF - α and MDA.

关 键 词：肺泡巨噬细胞 荧光指示剂 细胞内游离钙离子 肿瘤坏死因子-Α 丙二醛 

分 类 号：R686[医药卫生—骨科学]