沙地葡萄茎痘相关病毒RT-PCR检测  

Detection of Grapevine rupestris stem pitting associated virus by RT-PCR method

作　　者：董雅凤[1] 张尊平[1] 刘凤之[1] 王宝亮[1] 张少瑜[1] 

机构地区：[1]中国农业科学院果树研究所,辽宁兴城125100

出　　处：《果树学报》2007年第5期705-708,共4页Journal of Fruit Science

基　　金：辽宁省自然科学基金(20032139);人事部留学回国人员科技活动择优资助项目(农办人[2004]121号);辽宁省葫芦岛市科技攻关项目(04105B1601)

摘　　要：为建立快速、灵敏、可靠的沙地葡萄茎痘相关病毒(GRSPaV)检测方法,以总RNA为模板,采用2组GRSPaV特异性引物对29个品种52株葡萄样品进行RT-PCR检测,并对扩增产物进行了测序和分析。结果表明,从20个品种25株葡萄样品中检测到GRSPaV,平均带毒株率为48.1%。外壳蛋白基因片段引物F1/R1从25个样品中扩增到905bp的特异片段,复制酶基因片段引物F2/R2从20个样品中扩增到498bp的特异片段,表明GRSPaV外壳蛋白基因比复制酶基因更加保守,RT-PCR检测时采用F1/R1则更为适宜。PCR产物测序结果与GenBank中登录的GRSPaV序列比较,同源性为97.90%～98.11%。In order to establish rapid, sensitive and reliable detection method of GRSPaV, two pairs of primers of Grapevine rupestris stem pitting associated virus （GRSPaV） were used for detection of 52 grapevine samples of 29 cuhivars by RT-PCR with total RNA as templates. The PCR product was sequenced and analyzed. The results showed that 25 grapevine plants of 20 cuhivars were infected by GRSPaV and the infected percentage was 48.1%. The specific fragments of 905 bp were amplified from 25 samples by F1/R1 primers and 498 bp fragments from 20 samples by F2/R2 primers. The sequences of PCR products were aligned with that of GRSPaV from Genbank, the identity reached 97.90%-98.11%.

关 键 词：沙地葡萄茎痘相关病毒 RT-PCR 检测 

分 类 号：S663.1[农业科学—果树学]