高效液相色谱法测定双醋瑞因含量与有关物质  

Determination on Content and Related Substances of Diacerein with HPLC

作　　者：黄巧巧[1] 陈雪帆[1] 李会林[1] 

机构地区：[1]浙江省药品检验所,杭州310004

出　　处：《医药导报》2007年第9期1077-1078,共2页Herald of Medicine

摘　　要：目的建立高效液相色谱(HPLC)法测定双醋瑞因有关物质和含量。方法采用十八烷基硅烷键合硅胶为填充剂,乙腈-水(47:53,用醋酸调节pH值至2.7)为流动相,检测波长为254 nm。结果双醋瑞因与有关物质分离良好,线性范围为12～100μg.mL-1(r=1.000 0,n=7),最低检出量为0.5 ng。结论该法简单快速,专属性强,结果准确,可用作双醋瑞因含量测定和有关物质检查的方法。Objective To establish HPLC method for the determination on the content of diacerein and its related substances. Methods A C18 column was used , the mobile phase of acetonitrile-water （adjust pH to 2.7 with acetic acid） （47 ： 53） and the detection wavelength was 254 nm. Results Diacerein and related substances could be well isolated. The calibration curve was linear in the range of 12 - 100 μg·min^-1 with r = 1. 000 0 （ n = 7 ）. The detection limit was 0.5 ng. Conclusion This method is simple, rapid, accurate and suitable for the determination on the content of diaeerein and its related substances.

关 键 词：双醋瑞因 含量测定 有关物质 色谱法 高压液相 

分 类 号：R982[医药卫生—药品] R927.2[医药卫生—药学]