河朔荛花对山楂叶螨作用机制的初步研究  

Acarocidal Actions of Wikstroemiae chamedaphne against Tetranychus viennensis

作　　者：曹挥[1] 王有年[1] 张铁强[1] 赵莉蔺[2] 刘素琪[3] 师光禄[1] 

机构地区：[1]北京市农业应用新技术重点实验室,北京102206 [2]中国科学院动物研究所农业虫鼠害综合治理国家重点实验室,北京100080 [3]山西农业大学农学院,太谷030801

出　　处：《林业科学》2007年第8期65-70,共6页Scientia Silvae Sinicae

基　　金：北京市科委区县专项资金项目(2006);北京市都市农业学科群项目(XK100190553);北京市属市管高校人才强教计划(PXM2007-014207-044536);北京市属市管高校引进人才计划(PXM2007-014207-044538);山西省科技攻关项目(051044);山西省青年科学基金资助项目(20051036;2006021033);山西农业大学创新基金(2004002)

摘　　要：对河朔荛花活性成分最佳流分WCME-7和WCME-11处理后的雌成螨进行症状观察,初步推测它们可能通过影响山楂叶螨的神经传导和生殖、消化系统而导致雌成螨死亡。其中WCME-11击倒作用迅速,从12h开始山楂叶螨就进入了大量死亡期,具有比WCME-7更为强烈的症状表现和更全方位的作用方式。WCME-11能引起成螨体内的生理生化代谢,解毒酶类的活性被激活,Ca2+-ATP酶和乙酰胆碱酯酶活性被强烈抑制。Wikstroemiae chamedaphne has good acarocidal activity against Tetranychus viennensis,a serious pest of many crops including apple trees in China.Its active constitutes,WCME-7 and WCME-11,were separated and purified by column chromatograghy.The symptomal observiation showed that they had many actions,such as stomach poison,controlling development,controlling reproducing and so on.They maybe affect not only the nerve transmition but also digest,growing system.In contrast,WCME-11 showed more strong symptom phenomenon with more all-directions function method,knocked down more quickly.From 12 h,the mites began to enter death period on large.Function mechanism of WCME-11 was studied in the experiment.The results indicate that the WCME-11 could cause the fast reaction of the enzymes inside the mites.Before 8 h, the enzymes were changed.WCME-11 could cause mite to counteract poison by GSTS and CarE,Ca^2＋-ATP enzyme and AchE have been restrained strongly.

关 键 词：河朔荛花 山楂叶螨 作用机制 

分 类 号：S768.7[农业科学—森林保护学] S436.619[农业科学—林学]