Cloning,sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea  

作　　者：LU Jian-ping DUAN Zhi-bing LIU Tong-bao LIN Fu-cheng 

机构地区：[1]Department of Biology, School of Life Sciences, Zhejiang University, Hangzhou 310058, China [2]Biotechnology Institute, Zhejiang University, Hangzhou 310029, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2007年第9期661-665,共5页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：the National Natural Science Foundation of China (Nos. 30270049 and 30470064);the Natural Science Foundation of Zhejiang Province, China (No. Y304211)

摘　　要：The promoter of NAR gene in Magnaporthe grisea was isolated and sequenced. The promoter sequences contained the "TATA" box, the "CAAT" box, and binding sites for fungal regulatory proteins. Programs that predict promoter sequences in-dicated that promoter sequence lies between locations 430 and 857 of the NAR promoter fragment. GFP expression under the NAR promoter and NAR transcript analysis revealed that this promoter is activated primarily at the mycelial stage in the rice blast fungus and could be used to express native or extrinsic genes in the mycelia of the rice blast fungus.The promoter ofNAR gene in Magnaporthegrisea was isolated and sequenced. The promoter sequences contained the “TATA” box, the “CAAT” box, and binding sites for fungal regulatory proteins. Programs that predict promoter sequences indicated that promoter sequence lies between locations 430 and 857 of the NAR promoter fragment. GFP expression under the NAR promoter and NAR transcript analysis revealed that this promoter is activated primarily at the mycelial stage in the rice blast fungus and could be used to express native or extrinsic genes in the mycelia of the rice blast fungus.

关 键 词：Magnaporthe grisea PROMOTER NAR CLONE HYPHA 

分 类 号：S432[农业科学—植物病理学] Q933[农业科学—农业昆虫与害虫防治]