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作 者:ZHU Yong GAN Jun-jiang ZHANG Guo-liang YAO Bin ZHU Wen-jie MENG Qin
机构地区:[1]Shool of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China [2]School of Biological and Chemical Engineering, Zhejiang Uiversity of Science and Technology, Hangzhou 310032, China [3]Institute of Biological and Environmental Engineering, Zhejiang University of Technology Hangzhou 310014, China
出 处:《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》2007年第9期1514-1520,共7页浙江大学学报(英文版)A辑(应用物理与工程)
基 金:Project (No. 2006C100105) supported by the Ningbo Scientific Research Project, China
摘 要:In this work,rhamnolipid production was investigated using waste frying oil as the sole carbon source. By culture in shaking flasks,a naturally isolated strain synthesized rhamnolipid at concentration of 12.47 g/L and its mutant after treatment by UV light increased this productivity to 24.61 g/L. Fermentation was also conducted in a 50 L bioreactor and the productivity reached over 20 g/L. Hence,with a stable and high productive mutant strain,it could be feasible to reuse waste frying oil for rhamnolipid production on industrial scale.In this work, rhamnolipid production was investigated using waste frying oil as the sole carbon source. By culture in shaking flasks, a naturally isolated strain synthesized rhamnolipid at concentration of 12.47 g/L and its mutant after treatment by UV light increased this productivity to 24.61 g/L. Fermentation was also conducted in a 50 L bioreactor and the productivity reached over 20 g/L. Hence, with a stable and high productive mutant strain, it could be feasible to reuse waste frying oil for rhamnolipid production on industrial scale.
关 键 词:RHAMNOLIPID Pseudomonas aeruginosa BIOSURFACTANT Waste frying oil
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