右侧全肺切除致大鼠动力型肺动脉高压模型的建立  

Establishment of Hemodynamic Pulmonary Hypertension after Total Resection of Right Lung in Rats

作　　者：魏来[1] 赵强[1] 陈安清[1] 陆军[1] 姚瑞明[2] 贾剑国[2] 王克强[2] 邹云增[2] 

机构地区：[1]复旦大学附属中山医院心脏外科,上海200032 [2]上海市心血管病研究所中心实验室,上海200032

出　　处：《中国临床医学》2007年第4期482-484,共3页Chinese Journal of Clinical Medicine

摘　　要：目的:建立一种新的大鼠动力型肺动脉高压模型。方法:38只Wistar大鼠分为右肺全切组、假手术组和对照组。手术后4周测定大鼠体、肺动脉压,测算右心室重量与左心室加室间隔重量的比值[RV/(LV+S)],检测肺血管显微形态学指标—肺肌型小动脉相对中膜面积(RMA)及肺肌型小动脉占肺小血管百分比(SMA%)。结果:右肺全切组大鼠存活率66.7%(12/ 18),3组大鼠体动脉收缩压无显著差异,右肺全切组肺动脉收缩压、RV/(LV+S)、RMA和SMA%均明显高于假手术组和对照组,而假手术组和对照组之间各指标无显著差异。结论:大鼠右侧全肺切除法可以在较短时间(4周)内建立较明显的动力型肺动脉高压,操作相对简便,动物死亡率较低。Objective：To establish a new model of hemodynamic pulmonary hypertension in rats. Methods： Sixty-eight Wistar rats were randomly divided into group A（total resection of right lung）, group B（sham operated） and group C（control）. Four weeks later, the systolic systemic artery pressure（sSAP） and systolic pulmonary artery pressure（sPAP） were measured. The ratio of right ventricular mass to left ventricular plus septal mass [RV/（LV ＋ S）] was detected. The relative medial area （RMA） of small pulmonary muscularized arteries（SMA） and the percentage of SMA to all the small pulmonary vessels （SMA%） were measured and calculated. Results： Four weeks after operation, 66.7% rats survived （12/18） in group A. There was no difference in sSAP among the three groups（P〉0.05）. Group A had obviously higher sPAP, RV/（LV ＋ S）, RMA and SMA% than the other two groups（P〈0.01）. There was no difference between group B and C in all the indexes（P〉0.05）. Conclusion： Hemodynamic pulmonary hypertension can be quickly induced four weeks after total resection of right lung. This method was easily performed and the mortality of rats was relatively low.

关 键 词：肺切除 肺动脉高压 动物模型 大鼠 

分 类 号：R655.3[医药卫生—外科学]