乌司他丁在梗阻性黄疸时保护肠粘膜屏障功能的实验研究  

Effect of Urinastatin on Protection of Intestinal Mucosa of Rats with Obstructive Jaundice

作　　者：赵加应[1] 蔡元坤[1] 程志俭[1] 廖晓寰[2] 张杰[3] 徐火根[2] 章清[2] 何涛[1] 徐宏智[1] 潘杰[1] 

机构地区：[1]复旦大学附属上海市第五人民医院普外科,200240 [2]复旦大学附属上海市第五人民医院检验科,200240 [3]复旦大学附属上海市第五人民医院病理科,200240

出　　处：《中国临床医学》2007年第4期524-526,共3页Chinese Journal of Clinical Medicine

摘　　要：目的:研究乌司他丁(UTI)在梗阻性黄疸大鼠模型中保护肠粘膜屏障功能的作用。方法:将56只SD大鼠随机分为假手术组(sham)、阻黄组(OJ)、治疗组(UTI),制模成功后治疗组从尾静脉注射UTI 1 mL(1万U·只^(-1)),1次·d^(-1)。UTI3、UTI6和UTI9组分别连续用3d、6d和9d后处死,sham组、OJ组同期尾静脉注射等量生理盐水后处死;观察一般情况、下腔静脉采血测定血浆内毒素(ET)、TNF-α、WBC计数、细菌易位率、肝功能(ALT、SB),及取肝、小肠标本作病理分析。结果:UTI在治疗梗阻性黄疸大鼠时,与OJ比较,UTI组白细胞(WBC)、谷丙转氨酸(ALT)、总胆红素(SB)、内毒素(ET)、肿瘤坏死因子-α(TNF-α)、细菌易位率、腹水、伤口感染、出血、肝淤胆、肝细胞坏死程度、小肠粘膜水肿、糜烂、萎缩、炎性细胞浸润程度等明显降低。结论:UTI在治疗梗阻性黄疸大鼠时对保护肝功能、抑制全身炎症反应、降低肠道细菌易位率、保护肠粘膜屏障有一定的作用。Objective：To investigate the effat of on UTI of intestinal mucosa of rats with obstructive jaundice. Methods：Fifty- six SD rats were randomized into the Sham group, OJ group and UTI group. After the establishment of the model, the rats were injected 1 ml/10 000U UTI, one time for every day. After 3d,6d and 9d the rats of Group UTI3, UTI6 and UTI9 were killed separately, The Sham group and OJ group were killed by injecteng saline at the same time. Then the symptooms were watched, the blood was collected from inferior vena cava to detect the content of ET, TNF-α,WBC, the translocation rate of bacteria,ALT and SB. The specimen of liver and small intestine were collected to make the pathological analysis. Results： Compared with OJ group, the contents of WBQ, ALT, SB, ET, TNF-α, the transloeation rate of bacteria, aseites, wound infection, hemorrhage, cholestasis, necrosis of liver cells, the edema, erosion, atrophy and infiltration of inflammatory cell of intestinal mucosa of UTI group were all notabaly lowered down. Conclusion： UTI can protect the liver, inhibit the inflammatory reaction, lower down the translocation rate of bacteria and protect the defence function of intestinal mucosa when treating the rat model of Obstructive jaundice.

关 键 词：乌司他丁 梗阻性黄疸 肠粘膜屏障功能 肠道细菌易位 

分 类 号：R977.6[医药卫生—药品]