超排卵治疗过程中卵泡液促性腺激素、雌二醇和胰岛素样生长因子水平检测  

Determination of follicular gonadotropic hormone,estradiol,and insulin-like growth factor-I during superovulation

作　　者：铁新琴[1] 贾莉婷[1] 杨如镜[1] 

机构地区：[1]郑州大学第三附属医院生殖医学中心,郑州450052

出　　处：《郑州大学学报（医学版）》2007年第5期932-934,共3页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:检测超排卵治疗过程中卵泡液内某些激素以及生长因子的水平,探讨它们与受精后胚胎发育潜能的关系。方法:接受辅助生殖技术治疗的患者,根据胚胎发育状况及治疗结局分为妊娠组、正常未孕组和胚胎质量较差组,超促排卵后在超声引导下经阴道穿刺获得卵泡液,用放射免疫法测定卵泡液中促卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、孕酮(P)、生长激素(GH)以及胰岛素样生长因子1(IGF-1)的水平,比较其与不同胚胎发育状况及治疗结局之间的关系。结果:妊娠组及胚胎发育正常但未妊娠组E2水平明显高于胚胎质量较差组(P<0.05);妊娠组IGF-1水平明显高于胚胎质量较差组(P<0.05),正常未孕组居于2者之间,但差异无统计学意义;FSH、LH、P及GH水平在以上3组中差异无统计学意义。结论:超促排卵时卵泡液中E2及IGF-1可能与卵子受精后胚胎发育的潜能有关,测定其水平有助于评估辅助生殖技术治疗后的结局。Aim ： To determine the concentration of hormones and growth factors in follicular fluid and their effects on embryonic development potential in-vitro fertilization embryo transplantation （IVF-ET） treatment cycle. Methods： The concentrations of follicle stimulating hormone （ FSH ） , luteinizing hormone （ LH ） , estradiol （ E2 ） , progestogen （ P ） , growth hormone（GH） and insulin-like growth factor-Ⅰ（IGF-I） in follicular fluid obtained from patients undergoing IVF-ET were measured by radioimmunoassay. Results： The concentration of E2 was significantly higher in pregnant group and the group with normal embryonic morphology but no pregnancy compared with the group with poor embryonic morphology （P 〈 0. 05 ）. The concentration of IGF-Ⅰ was higher in pregnant group compared with the group with poor embryonic morphology （P 〈 0. 05）. Concentrations of FSH, LH, P and GH were similar in the three groups. Conclusion ： The concentrations of E2 and IGF-Ⅰ in follicular fluid during superovulation may be associated with the embryonic development potential. They may serve to assess the effect of IVF-ET.

关 键 词：胚胎发育 体外受精-胚胎移植 雌二醇 孕酮 生长激素 胰岛素样生长因子 

分 类 号：R711[医药卫生—妇产科学]