紫外分光光度法测定白芍总苷的含量  

Determination of paeoniflorin in Paeonia lactiflora Pall by UV spectrophotometry

作　　者：吴英华[1] 任凤莲[2] 蔡小华[1] 季怀萍[1] 瞿白露[2] 

机构地区：[1]怀化医学高等专科学校公共课部,湖南怀化418000 [2]中南大学化学化工学院,湖南长沙410083

出　　处：《应用化工》2007年第8期822-824,共3页Applied Chemical Industry

基　　金：湖南省教育厅青年基金项目(06B075)

摘　　要：研究了白芍总皂苷的紫外吸收特性,建立了以苯甲酸为对照品,用紫外分光光度法测定白芍总皂苷含量的方法。方法的回归方程为：Y=0.003 24＋0.107 63c,相关系数0.998 85,加标回收率为95.7%-102.0%,RSD=2.57%（n=9）。方法简便易行、快速、准确。The UV-absorption characteristics of the paeoniflorin in Paeonia lactiflora Pall were discussed, and the UV-absorption method for determination of total paeoniflorin with benzoic acid as control was developed. The regression equation was Y = 0. 003 24 ＋ 0. 107 63 c with a correlation coefficient of 0. 998 85. The standard addition recovery of elements was 95.7% - 102.0% and RSD was 2.57% （ n = 9）. This method was simple, fast and accurate.

关 键 词：白芍 白芍总苷 苯甲酸 分光光度法 

分 类 号：R282.71[医药卫生—中药学]