高迁移率族蛋白B1诱导小鼠腹腔巨噬细胞凋亡  

High Mobility Group Box-1 Protein Induces Apoptosis in Mouse Peritoneal Macrophages

作　　者：刘峰[1] 姚咏明[1] 董宁[1] 徐珊[1] 盛志勇[1] 

机构地区：[1]解放军总医院第一附属医院全军烧伤研究所基础部,北京100037

出　　处：《中国医学科学院学报》2007年第4期488-492,I0001,共6页Acta Academiae Medicinae Sinicae

基　　金：国家重点基础研究发展规划项目(2005CB522602);国家杰出青年科学基金(30125020);国家自然科学基金(30672178)~~

摘　　要：目的观察高迁移率族蛋白B1(HMGB1)对小鼠腹腔巨噬细胞凋亡的影响。方法分离培养小鼠腹腔巨噬细胞,经不同浓度的HMGB1(10、100、1000、10000ng/ml)作用24h,或以10000ng/ml的HMGB1作用不同时间(6、12、24、48h)后,以连接素Ⅴ/放线菌素D双染法,采用流式细胞术和激光扫描共聚焦显微镜检测巨噬细胞凋亡情况。结果经不同浓度的HMGB1刺激24h,其中10000ng/ml组HMGB1诱导巨噬细胞凋亡的作用最强,为(39.84±5.98)%,与对照组(4.49±1.72)%比较,差异具有显著性(P<0.01)。以10000ng/ml的HMGB1作用巨噬细胞不同时间,其中24h组凋亡率发生最高,为(38.30±6.95)%,显著高于对照组(P<0.01)。结论HMGB1可诱导小鼠腹腔巨噬细胞凋亡。Objective To investigate the effect of high mobility group box-1 protein （ HMGB1 ） on apoptosis of macrophages in mice. Methods The peritoneal macrophages were obtained from female BALB/c mice. After exposure to different doses of HMGB1 （10, 100, 1 000, and 10 000 ng/ml） for 24 hours, or exposure to 10 000 ng/ml for different time （6, 12, 24, and 48 hours）, cells were stained with Annexin V-PE and 7-amino-actinomycin-D （7-AAD）, then determined by flow cytometry and laser scanning confocal microscopy, respectively. Results Exposed to HMGB1 for 24 hours resulted in a dose-dependent induction of apoptosis, with increased levels of apoptosis observed at 100, 1 000, and 10 000 ng/ml compared with controls [（12.91 ±3.89）%, （18.76±3.41）%, and （39.84±5.98）%, respectively, vs. （4.49±1.72）%]. Exposed to HMGB1 at 10 000 ng/ml for 6, 12, 24, or 48 hours showed a time-dependent increase in apoptosis rate [ （18.33 ±4.60）%, （25.02 ±5.27）%, （38.30 ±6.95）%, and （21.81 ±7.96）% ]. The percentage of apoptotic cells markedly increased with a peak value at 24 hours after HMGB1 stimulation （ P 〈 0. 01 ）. Conclusion HMGB1 has a proapoptotic effect on murine peritoneal macrophages.

关 键 词：高迁移率族蛋白B1 凋亡 巨噬细胞 

分 类 号：R392.12[医药卫生—免疫学]