树突状细胞在介导创伤后迟发型超敏反应受抑中的作用  

Roles of Dendritic Cells in Mediating Decreased Delayed Type Hypersensitivity Responses after Trauma

作　　者：王震平[1] 梁华平[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所一室创伤,烧伤与复合伤国家重点实验室,重庆400042

出　　处：《中国医学科学院学报》2007年第4期501-505,共5页Acta Academiae Medicinae Sinicae

基　　金：国家重点基础研究发展计划项目(973计划)(2005CB522602);创伤;烧伤与复合伤国家重点实验室开放基金(2006A-2)~~

摘　　要：目的探讨失血合并闭合性骨折对小鼠迟发型超敏(DTH)反应的影响。方法致伤后分别用2,4-二硝基氟苯(DNFB)和异硫氰酸荧光素(FITC)涂抹小鼠腹部诱导DTH反应,用流式细胞术分析腹股沟淋巴结内FITC阳性细胞和树突状细胞(DC),并测定DNFB致敏24h后淋巴结细胞过继性转移DTH反应的能力。结果创伤小鼠的DTH反应显著低于正常小鼠(P<0.01)。流式细胞分析表明,创伤小鼠腹股沟淋巴结中的FITC阳性细胞、携带FITC的DC以及携带FITC的成熟DC的百分含量均显著低于正常小鼠(P<0.01)。DNFB致敏24h后淋巴结细胞过继性转移DTH反应的能力显著降低(P<0.01)。结论失血合并闭合性骨折导致小鼠DTH反应低下,这与创伤后DC向引流淋巴结内细胞递呈抗原的能力显著下降密切相关。Objective To study the effects of hemorrhage combined with closed fracture on delayed type hypersensitivity （DTH） responses in mice and to explore the relevant mechanisms. Methods DTH responses were induced with 2, 4-dinitro-1-fluorobenzene （DNFB） or fluorescein isothiocyanate （FITC） skin painting after injury, and single cell suspensions from pooled inguinal lymph nodes were analyzed by flow cytometry for FITC^＋ cells and dendritic cells （DC）. The ability of cells from pooled inguinal lymph nodes was tested 24 hours after skin painting with DNFB in transfelTing sensitization for DTH to DNFB. Results The DTH responses after injury decreased significantly compared with that of sham-injured mice （P 〈 0.01 ）. Flow cytometry showed that FITC^＋ cells, FITC ^＋/CD11 c ^＋ cells, and FITC ^＋/CD11 c ^＋ / major histocompatibility complex Ⅱ^＋ cells were all significantly decreased after trauma （ P 〈 0. 01 ）. The ability of cells to transfer sensitization for DTH to DNFB also declined （ P 〈 0. 01 ）. Conclusion Hemorrhage combined with closed fracture decreases the DTH responses in mice, which may be attributed to the reduced antigen-presenting capacity of DC in the injured mice.

关 键 词：创伤 迟发型超敏反应 树突状细胞 流式细胞分析 

分 类 号：R392.12[医药卫生—免疫学]