Bioleaching of chalcopyrite by mixed culture of moderately thermophilic microorganisms  

作　　者：邬长斌 曾伟民 周洪波 符波 黄菊芳 邱冠周 王淀佐 

机构地区：[1]China Ocean Mineral Resources Research and Development Association [2]School of Resource Processing and Bioengineering, Central South University [3]Key Laboratory of Biometallurgy, Ministry of Education

出　　处：《Journal of Central South University of Technology》2007年第4期474-478,共5页中南工业大学学报（英文版）

基　　金：Project(50621063, 40646029) supported by the National Natural Science Foundation of China;Project (2004CB619204) supported by the National Basic Research Program

摘　　要：A mixed culture of moderately thermophilic microorganisms was enriched from acid mine drainages(AMDs)samples collected from several sulphide mines in China,and the bioleaching of chalcopyrite was conducted both in shake flask and bioreactor.The results show that in the shake flask,the mixture can tolerate 50 g/L chalcopyrite after being acclimated to gradually increased concentrations of chalcopyrite.The copper extraction increases obviously in bioleaching of chalcopyrite with moderately thermophilic microorganisms supplemented with 0.4 g/L yeast extract at 180 r/min,74% copper can be extracted in the pulp of 50 g/L chalcopyrite after 20 d.Compared with copper extractions of mesophilic culture,unacclimated culture and acclimated culture without addition of yeast extract,that of accliniated culture with addition of yeast extract is increased by 53%,44% and 16%,respectively.In a completely stirred tank reactor,the mass fraction of copper and total iron extraction reach up to 81% and 56%,respectively.The results also indicate that it is necessary to add a large amount of acid to the pulp to extract copper from chalcopyrite effectively.A mixed culture of moderately thermophilic microorganisms was enriched from acid mine drainages（AMDs） samples collected from several sulphide mines in China, and the bioleaching of chalcopyrite was conducted both in shake flask and bioreactor The results show that in the shake flask, the mixture can tolerate 50 g/L chalcopyrite after being acclimated to gradually increased concentrations of chalcopyrite. The copper extraction increases obviously in bioleaching of chalcopyrite with moderately thermophilic microorganisms supplemented with 0.4 g/L yeast extract at 180 r/min, 74% copper can be extracted in the pulp of 50 g/L chalcopyrite after 20 d. Compared with copper extractions of mesophilic culture, unacclimated culture and acclimated culture without addition of yeast extract, that of accliniated culture with addition of yeast extract is increased by 53%, 44% and 16%, respectively. In a completely stirred tank reactor, the mass fraction of copper and total iron extraction reach up to 81% and 56%, respectively. The results also indicate that it is necessary to add a large amount of acid to the pulp to extract copper from chalcopyrite effectively.

关 键 词：BIOLEACHING moderately thermophilic microorganisms ACCLIMATION CHALCOPYRITE shake flask experiment stirred tank reactor 

分 类 号：TD952[矿业工程—选矿]