Lipo-PGE_1对大鼠缺血-再灌注损伤压疮模型的干预作用  

Interventional Effects of Lipo-PGE_1 on the Pressure Ulcers with Ischemia-reperfusion Injury in Rats

作　　者：杨晔琴[1] 姜丽萍[1] 蔡福满[1] 韦泾云[1] 

机构地区：[1]温州医学院护理学院,浙江温州325035

出　　处：《护理学杂志》2007年第17期10-12,共3页

摘　　要：目的探讨脂质载体前列腺素E1(Lipo-PGE1)对皮肤缺血-再灌注损伤压疮大鼠模型的干预作用,为压疮的防治提供依据。方法将24只大鼠随机分成缺血组(I组)、缺血-再灌注组(IR组)、Lipo-PGE1干预组(PGE1组)各8只。三组均于压疮装置上受压2h,I组解除受压后立即处死;IR组、PGE1组解除受压前10min分别于尾静脉注射生理盐水、Lipo-PGE1,解除受压4h时处死。结果与I组、PGE1组比较,IR组SOD活性显著降低,MDA、LDH、ET-1、NO含量显著增加(均P<0.05)。而PGE1组各项指标与I组比较,差异无显著性意义(均P>0.05)。结论缺血-再灌注损伤在压疮形成中发挥作用,Lipo-PGE1能有效拮抗氧自由基对皮肤的损伤,减轻皮肤缺血-再灌注损伤。Objective To evaluate the effects of Lipo-PGE1 on the pressure ulcers involved ischemia-reperfusion injury in rats. Methods twenty-four rats were classified into 3 groups randomly： the ischemic group （group Ⅰ） ; the ischemiareperfusion group （group IR） ;Lipo-PGE1 treated group （group PGE1 ）. All rats were subjected to decubitus compression model for 2 hours. Group Ⅰ were sacrificed right after decompression, while group IR and group PGE1 were treated with injection of normal saline and Lipo-PGE1 respectively 10 minutes before decompression and sacrificed 4 hours after decompression. The activity levels of superoxide dismutase （SOD）, lactic dehydrogenase （LDH） and the contents of malondialdehyde （MDA）, endothelin-1 （ET-1） and nitric oxide （NO） were all measured. Results The activity level of SOD in group IR was lower than that in group I and group PGE1 ; while the contents of MDA, LDH, ET-1 and NO were significantty increased（all P〈0.05）. There were no significant differeces of the indexes between group PGE1 and group Ⅰ （all P〉0.05）. Conclusion Ischemia-reperfusion injury played a vital role in the development of pressure ulcer. Lipo-PGE1 can be effectively against the skin injury by oxygen free radical, and relieve the ischemia-reperfusion injury of skin.

关 键 词：压疮 前列腺素E1 缺血-再灌注损伤 动物实验研究 

分 类 号：R47[医药卫生—护理学] R632.1[医药卫生—临床医学]