透明质酸圆窗灌注对豚鼠内耳功能和形态学的影响  

Effect of hyaluronan perfusion through the round window on the function and morphology of the inner ear in guinea pigs

作　　者：殷团芳[1] 卢永德[1] 陈忠[1] 任基浩[1] 谢鼎华[1] 杨新明[1] 谭薇薇 祝道桂[1] 

机构地区：[1]湖南医科大学第二临床学院耳鼻咽喉科教研室

出　　处：《中华耳鼻咽喉科杂志》1997年第2期92-94,共3页Chinese Journal of Otorhinolaryngology

摘　　要：为探索透明质酸中耳应用对内耳的影响，以２６只健康豚鼠于左耳用２％透明质酸圆窗灌注前和灌注后５、１４、２８天检测前庭和听觉功能，并行基底膜铺片及颞骨火棉胶包埋连续切片。发现２％透明质酸圆窗灌注后５天，滤波短声０．２５～１０ｋＨｚ诱发的听神经动作电位（ＡＰ）阈值均提高（Ｐ＜０．０５），术后１４天在１、２、４ｋＨｚＡＰ阈值明显改善（Ｐ＜０．０５），术后２８天均恢复至术前水平（Ｐ＞０．０５），灌注前后ＥＮＧ眼震时间无明显变化（Ｐ＞０．０５）。耳蜗Ｃｏｒｔｉ器和前庭终器组织学检查无明显损害。结论：２％透明质酸圆窗灌注对内耳听觉功能有暂时性影响，未见内耳组织学改变，透明质酸具有缓慢渗透脱水作用，可作为药物缓释体用于中耳及内耳手术。In order to study the effect of the hyaluronan (HA) on the inner ear, twenty six guinea pigs were divided into experimental and control groups, the experimental group were perfused with 2%HA 0.2 ml through the left round window. Auditory and vestibular function were investigated before and 5, 14, 28 days after perfusion. The threshold of action potential (AP) response to filtered clicks (0.25～10 kHz) were elevated at 5 days and were improved (at 1～4 kHz) at 14 days after perfusion ( P <0.05). At 28 days all AP response thresholds had returned to normal ( P > 0.05). The nystagmus duration induced by caloric test (ENG) had no obviously change comparing with that of preoperation( P >0.05). All guinea pigs were sacrificed 28 days after operation. The histology of inner ear were examined by means of membranous labyrinth mapping and temporal bone section after celloidin embedding. The morphology of Corti's and vestibular organs were not significantly damaged after perfusion. In conclusion: 1) 2%HA caused reversible and temporary changes in auditory function of inner ear; 2) 2%HA lacked ototoxic adverse effects, whereas exerted considerable osmotic effects and a slow dehydration of inner ears.

关 键 词：透明质酸 迷路 前庭功能 诱发反应 

分 类 号：R322.923[医药卫生—人体解剖和组织胚胎学]