糖尿病早期大鼠视网膜微血管改变的形态学研究  

An observation on early morphological changhes in retinal microvascular vessels in diabetic rats

作　　者：杨婴[1] 何守志[1] 刘艳芳[1] 

机构地区：[1]解放军总医院眼科

出　　处：《中华眼科杂志》1997年第3期230-232,共3页Chinese Journal of Ophthalmology

摘　　要：目的研究糖尿病早期大鼠视网膜微循环的形态学改变及白细胞在糖尿病视网膜病变（ｄｉａ－ｂｅｔｉｃｒｅｔｉｎｏｐａｔｈｙ，ＤＲ）微循环障碍中的作用。方法雄性成年Ｗｉｓｔａｒ大鼠１２只，随机分为正常对照组、链脲佐菌素（ｓｔｒｅｐｔｏｚｏｔｏｃｉｎ，ＳＴＺ）糖尿病大鼠３、６个月组各４只。动物活体灌注固定，视网膜消化铺片，光镜观察，计数周细胞并行统计分析。结果糖尿病大鼠６个月组视网膜毛细血管周细胞数明显减少，并见毛细血管有白细胞栓塞。结论大鼠注射ＳＴＺ后６个月出现ＤＲ的早期改变，白细胞栓塞毛细血管形成ＤＲ微循环障碍。Objictive To investigate the morphological changes of retinal microcirculation in streptozotocin (STZ) diabetic rats and the contribution of leukocyte to diabetic retinopathy(DR).Methods 12 male Wistar rats were randomly divided into normal control group, 3 month group and 6 month group of STZ diabetic rats.Each group was perfused with 4% paraformaldehyde and fixed.Rat retina digest preparations were observed by light microscope,and the number of pericyte was counted.Results The decrease of pericytes in STZ diabetic rats was remarkable compared with that of norm alcontrol at 6 months.At 6 months,STZ diabetic rat retinal digest preparations showed that some of the capillaries were blocked by leukocytes.Conclusion After injection of STZ in rats for 6 months,early morphological changes of DR were observed,and leukocytes played a potential role in the pathogenesis of DR.

关 键 词：糖尿病 微循环 白细胞 视网膜疾病 

分 类 号：R587.1[医药卫生—内分泌] R774.1[医药卫生—内科学]