龙胆抗病毒有效部位RG3-1体外抑制呼吸道合胞病毒作用的研究  

Antiviral active compound(RG3-1) from radix gentianae and its anti-RSV activity in vitro

作　　者：陈振华[1] 李洪源[1] 谢志平[1] 张建军[1] 李鑫[1] 王吉锡[1] 朴英爱 周婧瑜[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,黑龙江哈尔滨150081 [2]黑龙江药品检验所,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2007年第4期344-346,348,共4页Journal of Harbin Medical University

摘　　要：目的探讨龙胆抗病毒有效部位(RG3-1)体外抑制呼吸道合胞病毒(RSV)的作用。方法采用煎煮法、二氯甲烷萃取法和柱层析法分离提取RG3-1。以病毒唑为阳性对照药物,采用细胞病变抑制试验观察RG3-1对RSV的抑制作用。结果RG3-1半数中毒浓度(TC50)为5.29mg/mL,抑制RSV的半数有效浓度EC50为262.95μg/mL,治疗指数(TI)为20.12;RG3-1对RSV的抑制存在量效关系;RG3-1具有直接灭活RSV的作用;在感染后至少10h以内给药,RG3-1能够有效的抑制RSV复制。结论RG3-1在HeLa细胞中对RSV有明显的抑制作用。Objective To study the anti-respiratory syncytial virus （RSV） effect of an compound （RG3-1） from radix gentianae in vitro .Methods RG3-1 was extracted from radix gentianae by hot water, dichloromethane, and column chromatography. The cytotoxicity and antiviral action of RG3-1 were tested by cytopathic effect （CPE） inhibition assay,and ribavirin as the positive control drug. Results It showed good inhibitory effect against RSV with the medium cytotoxicity concentration （TCso） 5.29mg/mL, the medium effective concentration （ECso） 262.95μg/mL,and the treatment index（TI） 20.12. RG3-1 was effective antiviral agent for RSV in a concentration-dependent manner. RG3-1 also displayed direct inactivation on RSV. It also exhibited obvious inhibition on RSV replications when RG3-1 was added in 10h after infection （P 〈 0.01 ）. Conclusion RG3-1 can significantly inhibit RSV replications in HeLa cells.

关 键 词：龙胆 呼吸道合胞病毒 抗病毒作用 

分 类 号：R373.1[医药卫生—病原生物学] R285.5[医药卫生—基础医学]