卡特兰叶片原生质体分离条件的研究  

Research of the Optimum Conditions for Leaf Protoplast Isolation of Cattleya hybrida

作　　者：陈泽雄[1,2] 刘奕清[1,2] 丁茂倩[1,2] 

机构地区：[1]重庆文理学院生命科学系 [2]重庆园林花卉工程研究中心,重庆永川402168

出　　处：《西南大学学报（自然科学版）》2007年第8期97-101,共5页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市教委重大平台建设资助项目(GCZX0713);重庆文理学院重点科研资助项目

摘　　要：试验以卡特兰叶片组织为材料,研究了预处理时间、酶液组合、酶解时间、酶液中甘露醇含量等不同因素对其原生质体分离的影响.结果表明,以1%的纤维素酶+0.3%的果胶酶为混合酶液,11%的甘露醇为渗透压稳定剂,在25±1℃的条件下,pH为5.8的酶液组合中酶解8 h,获得的原生质体产量最高,可达8.9×106/g鲜重,存活率高达91.2%.The leaves of Cattleya hybrida were used as experiment materials and the influences of pretreatment time, enzyme combinations, digestion time and mannitol concentrations in the mixed enzyme solution on protoplast isolation from them were studied. The results showed that the highest yield （8. 9 × 10^6/g FW） and viability （91.2^） were obtained by treating the leaves with a mixture of celluase Onozuka R-10 （w=1%） ＋ macerozyme R-10 （w=0.3M） for 8h at the temperature of 25±1℃ with 11% mannitol as the osmotic pressure stabilizer.

关 键 词：卡特兰 叶片 原生质体分离 

分 类 号：Q949.718.43[生物学—植物学]