无糖培养条件下大型组培箱内CO_2变化规律及对组培苗的影响  

Effect of CO_2 concentration transformation rule in the sugar-free large tissue culture box on the tissue-culture plantlet

作　　者：曲英华[1] 周炜[1] 李艳[1] 邓健[1] 

机构地区：[1]中国农业大学农业部设施农业生物环境工程重点开放实验室,北京100083

出　　处：《农业工程学报》2007年第8期216-221,共6页Transactions of the Chinese Society of Agricultural Engineering

基　　金：北京市教育委员会都市农业学科群建设项目资助(XK100190553);北京市教育委员会共建项目建设计划资助(XK100190550)

摘　　要：该文采用中国农业大学农业部设施农业生物环境工程重点开放实验室研制的设有组培微环境实时监控系统的大型组培箱,分别对矮牵牛、菊花和番茄组培苗移栽后箱体内CO2浓度的变化规律及不同CO2增施浓度对无糖组培苗生长的影响进行了研究.试验表明：移栽后的当天,组培箱内的CO2浓度便开始下降,第2 d下降速度明显加快,均降至100 μL/L以下.在移栽后的第4～5 d,箱体内CO2浓度下降到35 μL/L左右后便不再下降,一直在30～40 μL/L之间波动.因此得出：无糖培养在组培苗移栽后的第2 d就应增施CO2,否则会直接影响组培苗的生长.在不同CO2增施浓度试验中,当光照度控制在80 μmol/（m^2·s）时,CO2浓度为（650±50）μL/L时培养出的组培苗生长状况最好.In this paper, the rule of CO2 concentrations and the effects of CO2 enrichment concentration on the growth of sugar-free culture plantlets in the culture box with real-time control system after transplanting petunia, chrysanthmum and tomato plantlet were investigated, which was developed by Key Lab of Agricultural Bio-envi- roment Engineering, Ministry of Agricultue, China Agricultural University. The day after transplanting, the CO2 concentration started to decrease, speeding up the rate of decline in the second day and it dropped below 100 μL/L. In the first 4～5 days after transplanting, CO2 conpensation dropped to between 30》40μL/L. So CO2 should be enriched on the second day after transplanting, otherwise, it would affect plantlet growth. In different CO2 enrichment tests, tissue-culture is the best at CO2 concentration of （650 ±50） μmol/mol under 80 μmol/（m^2 · s） Photosynthetic Photon Flux Density（PPFD）.

关 键 词：无糖组培 CO2浓度 组培苗 

分 类 号：Q813.12[生物学—生物工程]