博莱霉素静脉注射制备小鼠肺间质纤维化模型  

Establishment of a Mouse Model of Pulmonary Fibrosis by Intravenous Administration of Bleomycin

作　　者：张晓晔[1] 志村早苗[2] 增田辙[2] 齐藤宏树[2] 

机构地区：[1]中国医科大学盛京医院呼吸内科,沈阳110004 [2]东北大学医学部第一内科

出　　处：《中国实验动物学报》2007年第4期241-244,F0002,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金资助项目(3010007630570812);辽宁省自然科学基金资助项目(20042085)基金资助

摘　　要：目的经尾静脉一次性注射博莱霉素(BLM)复制小鼠肺间质纤维化动物模型,并观察模型的肺组织病理学变化。方法8周龄雌性C57BL/6小鼠66只,随机分为BLM80组18只、BLM150组19只、BLM300组19只和对照组10只,分别经尾静脉一次性注射BLM80、150、300 mg/kg和生理盐水。结果①BLM80组和BLM150组小鼠最低体重分别为BLM注射前的84%和65%。②BLM80组、BLM150组、BLM300组和对照组小鼠的生存率分别为:100%、43%、0和100%。③BLM150组BLM注射后14 d、28 d,右肺羟脯氨酸含量分别为738±46 nmol、886±83 nmol,与对照组(360±75 nmol)比较差异均有显著性意义(P<0.01)。④BLM150组小鼠BLM注射28 d,在胸膜下及血管周围形成广泛、稳定、明显的纤维化改变。BLM150组与BLM80和对照组比较,肺纤维化病理评分分别呈明显增高(P值均小于0.001)。结论C57BL/6小鼠经尾静脉一次性注射BLM150mg/kg可以制备肺间质纤维化动物模型。Objective To establish a C57BL/6 mouse model of pulmonary fibrosis with bleomycin and observe the pathologic changes of lungs in the model mice. Methods Sixty six C57BL/6 mice （female,8 wk of age, 17 to 20 g body weight） were randomly divided into BLM80 group, BLM150 group, BLM300 group and control group, which received intravenous injection of bleomycin in a dose of 80 mg/kg, 150mg/kg,300mg/kg and normal saline via the tail vein, respectively. Results （1） The mice lost 16 % and 35 % of their body weight after 80 mg/kg and 150 mg/kg bleomycin injection, respectively. （2） The survival rate of BLM80 group, BLM150 group, BLM300 group and control group was 100 %, 43 %, 0 and 100 %, respectively. （3） At 14 and 28 days after injection, the hydroxyproline content per lung in the BLM150 group was 738 ± 46 nmol and 886 ± 83 nmol, respectively, significantly increased than that in the control group （360 ± 75 nmol） （P 〈 0.01 ）. （4） At 28 days after bleomycin injection, fibrosis was widely and stably formed in the subpleura and perivascular area in the mouse lungs of BLM150 group. The pathological scores of pulmonary fibrosis in BLM150 group were significantly higher than those in BLM80 and normal groups （ P 〈 0.001, respectively）. Conclusions Pulmonary fibrosis model has been successfully established by intravenous injection of bleomycin once in a dose of 150 mg/kg in C57BL/6 mice.

关 键 词：小鼠 近交C57BL 博莱霉素 肺纤维化 模型 动物 

分 类 号：R563.1[医药卫生—呼吸系统]