诺氟沙星人工抗原的合成  

Synthesis of Norfloxacin Artificial Antigen

作　　者：于海峰[1] 谭建华[2] 孙靖[1] 张森[1] 李岩松[1] 

机构地区：[1]吉林大学农学部畜牧兽医学院,长春130062 [2]解放军军事医学科学院军事兽医研究所,长春130062

出　　处：《吉林农业大学学报》2007年第4期443-446,共4页Journal of Jilin Agricultural University

基　　金：国家自然科学基金(30571356)

摘　　要：采用碳二亚胺(EDC)法将诺氟沙星(NFLX)分别与载体蛋白BSA和OVA进行偶联。用于制备免疫抗原NFLX-BSA和包被抗原NFLX-OVA。通过对产物进行紫外扫描和SDS-PAGE,证明半抗原与载体偶联成功。以免疫原NFLX-BSA免疫BALB/C小鼠45 d后,通过间接ELISA方法检测效价达1:32 000。这一结果为进一步制备抗NFLX单克隆抗体提供了良好的免疫原。Using EDC, the norfloxacin （NFLX） was coupled with BSA and OVA to prepare immunogen NFLX-BSA and coating antigen NFLX-OVA. UV scanning and SDS-PAGE indicated that the antigens were successfully produced. BALB/C mouse had been immtmized with NFLX-BSA for 45 days and the titer testified by indirect ELISA from those mice was 1 ： 32 000. The present research supplied a good immune antigen for further preparation of monoclonal antibody to NFLX.

关 键 词：诺氟沙星 人工抗原 碳二亚胺 

分 类 号：R392.11[医药卫生—免疫学]