利用复合PCR结合DNA芯片的方法进行快速转基因事件检测  

Coupling Multiplex PCR with DNA Chips to Rapidly Detect and Identify Genetically Modified Events

作　　者：肖一争[1] 唐咏[1] 宛煜嵩[2] 金芜军[2] 

机构地区：[1]沈阳农业大学,沈阳110161 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《中国农业科技导报》2007年第4期101-107,共7页Journal of Agricultural Science and Technology

基　　金：国家"863"计划项目转基因食品安全性评价与转基因农产品检测方法研究(2004AA212222)资助

摘　　要：以我国批准进口用作加工原料的6种转基因玉米为材料,根据其外源基因、载体构建及插入位点,应用6对特异性引物及35S、NOS引物,设计并优化了复合PCR反应体系,对其进行转基因成分检测。同时尝试利用复合PCR与DNA芯片相结合,对不同转基因玉米混合样品进行检测,探索建立转基因事件快速检测和确认方法。A multiplex PCR system was designed and optimized with 35S and Nos primer and other 6 special primers which are designed according to inserted genes, plasmid constructions and inserted sites to detect 6 transgenic maize events which are permitted to import as product material by China, A primarily designed DNA chip coupled with the multiplex PCR system was also used to detect mixed GM maize samples, We attempted to construct a rapid GM maize events detection and confirmation method.

关 键 词：转基因玉米 复合PCR DNA芯片 

分 类 号：Q813[生物学—生物工程]