Immunolocalization of secretion complex in Streptococcus mutans  

作　　者：PING HU ZHUAN BIAN MING WEN FAN MIN NIE 

机构地区：[1]Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology , Wuhan University, P. R. China

出　　处：《Journal of Microbiology and Immunology》2007年第1期13-16,共4页中华微生物学和免疫学（英文版）

基　　金：This work was supported by National Natural Science Foundation of China(30670115).

摘　　要：Each secretion （See） machinery minimally consists of three integral inner membrane pro- teins SecYEG. In order to know the distribution pattern of the Sec complex in Streptococcus mutans, we explored the subcellular localization of SecY. The anti-secY antibody was examined on Western blots to evaluate specificity. An indirect post-embedding immunogold method was used to determine the subcellular localization of the SecY in the cytoplasmic membrane of the Streptococcus mutans GS-5. lmmunoblotting experiments showed that the anti-seeY antibody specifically recognized a single band of about 47.8 kDa. Immunogold electron microscopy image of section with anti-secY antibody revealed a single intense focus of gold particles at a discrete location on the cytoplasmic membrane of the Streptococcus mutans GS-5. SecY clustered to an asymmetric microdomain, which indicated for the first time that See complex presented a unisite on the cytoplasmic membrane of Streptococcus mutans.Each secretion(Sec)machinery minimally consists of three integral inner membrane pro- teins SecYEG.In order to know the distribution pattern of the Sec complex in Streptococcus mutans,we explored the subcellular localization of SecY.The anti-secY antibody was examined on Western blots to evaluate specificity.An indirect post-embedding immunogold method was used to determine the subcel- lular localization of the SecY in the cytoplasmic membrane of the Streptococcus mutans GS-5.Immuno- blotting experiments showed that the anti-secY antibody specifically recognized a single band of about 47.8 kDa.Immunogold electron microscopy image of section with anti-secY antibody revealed a single intense focus of gold particles at a discrete location on the cytoplasmic membrane of the Streptococcus mutans GS-5.SecY clustered to an asyrmnetric microdomain,which indicated for the first time that Sec complex presented a uni-site on the cytoplasmic membrane of Streptococcus mutans.

关 键 词：Streptococcus mutans Secretion complex SECY 

分 类 号：R392[医药卫生—免疫学]